SigD

• Description: RNA polymerase sigma factor SigD
  
  

Gene name	sigD
Synonyms	flaB
Essential	no
Product	RNA polymerase sigma factor SigD
Function	regulation of flagella, motility, chemotaxis and autolysis
Gene expression levels in SubtiExpress: sigD
Interactions involving this protein in SubtInteract: SigD
MW, pI	29 kDa, 5.167
Gene length, protein length	762 bp, 254 aa
Immediate neighbours	cheD, swrB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, sigma factors and their control, motility and chemotaxis

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16470

Phenotypes of a mutant

• Inactivation of alsR, sigD and sigW increases competitive fitness of Bacillus subtilis under non-sporulating conditions PubMed
• mucoid phenotype due to the overproduction of poly-gamma-glutamate (because of the loss of motA-motB expression) PubMed
• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

Database entries

• BsubCyc: BSU16470

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sigma-70 factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: interaction with FlgM inhibits SigD activity PubMed

• Interactions:
  • SigD-FlgM PubMed, SigD-(RpoB-RpoC)

• Localization:

Database entries

• BsubCyc: BSU16470

• Structure:

• UniProt: P10726

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: part of the fla-che operon
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • sigD-swrB PubMed

• Expression browser: sigD PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed
  • sigD: SigA PubMed

• Regulation: see fla-che operon

• Regulatory mechanism: see fla-che operon

• Additional information:

Biological materials

• Mutant:
  • 1A716 ( sigD::cat), PubMed, available at BGSC
  • DS6420 (marker-less in NCIB3610) PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• FLAG-tag construct: GP948 (ermC, based on pGP1087), available in the Stülke lab

• Antibody: