Difference between revisions of "FloA"

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* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
 +
** oligomerization depends on the glutamate-alanine repeats {{PubMed|25909364}}
 
** [[FloA]]-[[FtsH]] {{PubMed|22882210}}
 
** [[FloA]]-[[FtsH]] {{PubMed|22882210}}
 
** [[FloT]]-[[FloA]] {{PubMed|23651456}}
 
** [[FloT]]-[[FloA]] {{PubMed|23651456}}
 +
** [[FloA]]-[[PhoR]] {{PubMed|25909364}}
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
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** co-localizes with [[FloT]] in discrete foci in the membrane {{PubMed|20713508}}
 
** co-localizes with [[FloT]] in discrete foci in the membrane {{PubMed|20713508}}
 
** forms focal structure in the cytoplasma membrane {{PubMed|22753055}}
 
** forms focal structure in the cytoplasma membrane {{PubMed|22753055}}
 +
** 13 foci [[FloA]] of are detected in strain 3610 {{PubMed|25909364}}
 +
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Mutant:'''
 
* '''Mutant:'''
 +
** JS119 (3610 markerless), available in [[Daniel Lopez]]'s lab
 +
** DL1401 (168 [[ΔfloA]]::mls), available in [[Daniel Lopez]]'s lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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* '''GFP fusion:'''
 
* '''GFP fusion:'''
 +
** GK192 (3610 [[lacA]]::P[[floT-yfp]](mls)), available in [[Daniel Lopez]]'s lab
 +
** JS136 (3610 [[amyE::floT]]-gfp(spc)), available in [[Daniel Lopez]]'s lab
 +
** JS134 (3610 [[lacA::floA]]-mEos2 (mls)), available in [[Daniel Lopez]]'s lab
 +
** JS167 (3610 [[lacA::floA]]-PAmCherry (mls)), available in [[Daniel Lopez]]'s lab
  
−
* '''two-hybrid system:'''  
+
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Daniel Lopez]]'s lab
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 11:35, 30 April 2015

  • Description: flottilin-like protein (in addition to FloT), resistence protein (against sublancin), accessory role in resistance to cefuroxime

Gene name floA
Synonyms yqfA
Essential no
Product flottilin-like protein
Function resistance against sublancin
Gene expression levels in SubtiExpress: floA
Interactions involving this protein in SubtInteract: FloA
MW, pI 35 kDa, 4.917
Gene length, protein length 993 bp, 331 aa
Immediate neighbours yqfB, yqeZ
Sequences Protein DNA DNA_with_flanks
Genetic context
YqfA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YqfA expression.png















Categories containing this gene/protein

membrane dynamics, cell envelope stress proteins (controlled by SigM, V, W, X, Y), resistance against toxins/ antibiotics, membrane proteins

This gene is a member of the following regulons

SigW regulon

The gene

Basic information

  • Locus tag: BSU25380

Phenotypes of a mutant


Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: UPF0365 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:


Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 3975 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 3635 PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Daniel Lopez's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Marc Bramkamp, Daniel Lopez
Exploring the existence of lipid rafts in bacteria.
Microbiol Mol Biol Rev: 2015, 79(1);81-100
[PubMed:25652542] [WorldCat.org] [DOI] (I p)

Original publications

Johannes Schneider, Teresa Klein, Benjamin Mielich-Süss, Gudrun Koch, Christian Franke, Oscar P Kuipers, Ákos T Kovács, Markus Sauer, Daniel Lopez
Spatio-temporal remodeling of functional membrane microdomains organizes the signaling networks of a bacterium.
PLoS Genet: 2015, 11(4);e1005140
[PubMed:25909364] [WorldCat.org] [DOI] (I e)

Benjamin Mielich-Süss, Johannes Schneider, Daniel Lopez
Overproduction of flotillin influences cell differentiation and shape in Bacillus subtilis.
mBio: 2013, 4(6);e00719-13
[PubMed:24222488] [WorldCat.org] [DOI] (I e)

Juri Niño Bach, Marc Bramkamp
Flotillins functionally organize the bacterial membrane.
Mol Microbiol: 2013, 88(6);1205-17
[PubMed:23651456] [WorldCat.org] [DOI] (I p)

Ana Yepes, Johannes Schneider, Benjamin Mielich, Gudrun Koch, Juan-Carlos García-Betancur, Kumaran S Ramamurthi, Hera Vlamakis, Daniel López
The biofilm formation defect of a Bacillus subtilis flotillin-defective mutant involves the protease FtsH.
Mol Microbiol: 2012, 86(2);457-71
[PubMed:22882210] [WorldCat.org] [DOI] (I p)

Felix Dempwolff, Heiko M Möller, Peter L Graumann
Synthetic motility and cell shape defects associated with deletions of flotillin/reggie paralogs in Bacillus subtilis and interplay of these proteins with NfeD proteins.
J Bacteriol: 2012, 194(17);4652-61
[PubMed:22753055] [WorldCat.org] [DOI] (I p)

Yong Heon Lee, Anthony W Kingston, John D Helmann
Glutamate dehydrogenase affects resistance to cell wall antibiotics in Bacillus subtilis.
J Bacteriol: 2012, 194(5);993-1001
[PubMed:22178969] [WorldCat.org] [DOI] (I p)

Daniel López, Roberto Kolter
Functional microdomains in bacterial membranes.
Genes Dev: 2010, 24(17);1893-902
[PubMed:20713508] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Bronwyn G Butcher, John D Helmann
Identification of Bacillus subtilis sigma-dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli.
Mol Microbiol: 2006, 60(3);765-82
[PubMed:16629676] [WorldCat.org] [DOI] (P p)

Min Cao, Phil A Kobel, Maud M Morshedi, Ming Fang Winston Wu, Chris Paddon, John D Helmann
Defining the Bacillus subtilis sigma(W) regulon: a comparative analysis of promoter consensus search, run-off transcription/macroarray analysis (ROMA), and transcriptional profiling approaches.
J Mol Biol: 2002, 316(3);443-57
[PubMed:11866510] [WorldCat.org] [DOI] (P p)

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