CotA

• Description: laccase, bilirubin oxidase, spore coat protein (outer)
  
  

Gene name	cotA
Synonyms	pig
Essential	no
Product	laccase, bilirubin oxidase
Function	resistance of the spore
Gene expression levels in SubtiExpress: cotA
MW, pI	58 kDa, 5.89
Gene length, protein length	1539 bp, 513 aa
Immediate neighbours	yeaA, gabP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

GerE regulon, SigK regulon, Efp-dependent proteins

The gene

Basic information

• Locus tag: BSU06300

Database entries

• BsubCyc: BSU06300

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • outer spore coat, more abundant at the mother cell-distal pole of the forespore, localization depends on CotE PubMed

Database entries

• BsubCyc: BSU06300

• Structure: 2BHF (reduced form)

• UniProt: P07788

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotA PubMed

• Expression browser: cotA PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK, GerE) PubMed

• Regulatory mechanism:
  • GerE: transcription repression PubMed

• Additional information:
  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

• Mutant:
  • 1S101 ( cotA::cat), PubMed, available at BGSC
  • 1S134 ( cotA::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Peter T McKenney, Adam Driks, Patrick Eichenberger
The Bacillus subtilis endospore: assembly and functions of the multilayered coat.
Nat Rev Microbiol: 2013, 11(1);33-44
[PubMed:23202530] [WorldCat.org] [DOI] (I p)