Difference between revisions of "SacT"

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(Biological materials)
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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
 +
{{SubtiWiki regulon|[[DnaAregulon]]}}
  
 
=The [[SacT regulon]]: ''[[sacP]]-[[sacA]]-[[ywdA]]''=
 
=The [[SacT regulon]]: ''[[sacP]]-[[sacA]]-[[ywdA]]''=
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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[sacT]]'' {{PubMed|9353933}}
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* '''Operon:'''  
 +
** ''[[ywcI]]-[[sacT]]'' {{PubMed|22383849}}
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** ''[[sacT]]'' {{PubMed|9353933}}
  
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sacT_3906142_3906972_-1 sacT] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sacT_3906142_3906972_-1 sacT] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
+
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[DnaA]]: transcription repression {{PubMed|26020636}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
  
<pubmed>12107147 2163394 1577686 8702561 1279678, 21278164 </pubmed>
+
<pubmed>12107147 2163394 1577686 8702561 1279678, 21278164 26020636 22383849</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 09:33, 1 June 2015

Gene name sacT
Synonyms ipa-47d
Essential no
Product transcriptional antiterminator
Function regulation of sucrose utilization
Gene expression levels in SubtiExpress: sacT
Interactions involving this protein in SubtInteract: SacT
Metabolic function and regulation of this protein in SubtiPathways:
sacT
MW, pI 31 kDa, 5.587
Gene length, protein length 828 bp, 276 aa
Immediate neighbours ywcJ, ywcI
Sequences Protein DNA DNA_with_flanks
Genetic context
SacT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
500px















Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, RNA binding regulators, phosphoproteins

This gene is a member of the following regulons

DnaAregulon

The SacT regulon: sacP-sacA-ywdA

The gene

Basic information

  • Locus tag: BSU38070

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binding to the mRNA of the sacP-sacA operon, causes transcription antitermination (in presence of sucrose and absence of glucose)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
    • for expression, purification of both PRDs in E. coli with N-terminal His-tag, in pWH844: pGP166, available in Jörg Stülke's lab
    • for expression, purification of PRD-1 in E. coli with N-terminal His-tag, in pWH844: pGP426, available in Jörg Stülke's lab
    • for expression, purification of PRD-2 in E. coli with N-terminal His-tag, in pWH844: pGP427, available in Jörg Stülke's lab
    • for expression, purification of PRD-1 in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP439, available in Jörg Stülke's lab
    • for expression, purification of PRD-2 in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP440, available in Jörg Stülke's lab
    • for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP571, available in Jörg Stülke's lab
    • for expression of the RNA-binding domain in B. subtilis, in pBQ200: pGP446, available in Jörg Stülke's lab
    • for expression, purification of sacT-full length in B. subtilis with C-terminal Strep-tag, in pGP382: pGP1064, available in Jörg Stülke's lab
    • for expression, purification of sacT-full length in B. subtilis with N-terminal Strep-tag, in pGP380: pGP1068, available in Jörg Stülke's lab
  • lacZ fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References