RpsJ

• Description: ribosomal protein
  
  

Gene name	rpsJ
Synonyms	tetA
Essential	yes PubMed
Product	ribosomal protein S10 (BS13)
Function	translation
Gene expression levels in SubtiExpress: rpsJ
Interactions involving this protein in SubtInteract: RpsJ
MW, pI	11 kDa, 10.207
Gene length, protein length	306 bp, 102 aa
Immediate neighbours	ybaC, rplC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, universally conserved proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU01150

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU01150

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ribosomal protein S10P family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • located near the aminoacyl-tRNA site of the 30S ribosomal subunit PubMed

Database entries

• BsubCyc: BSU01150

• Structure:

• UniProt: P21471

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO PubMed

• Expression browser: rpsJ PubMed

• Sigma factor:
  • SigA two promoters, about 140bp and 200bp upstream of the start codon PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 8159 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 13952 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:
  • pGP605 (in pAC6), available in Jörg Stülke's lab
  • pGP2201 (in pAC7), available in Jörg Stülke's lab
  • pGP2439 (in pAC7), available in Jörg Stülke's lab
  • pGP2472 (in pAC5), available in Jörg Stülke's lab
  • pGP2473 (in pAC6), available in Jörg Stülke's lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Tom Coenye, Peter Vandamme
Organisation of the S10, spc and alpha ribosomal protein gene clusters in prokaryotic genomes.
FEMS Microbiol Lett: 2005, 242(1);117-26
[PubMed:15621428] [WorldCat.org] [DOI] (P p)