CitA

• Description: minor citrate synthase
  
  

Gene name	citA
Synonyms	citA1
Essential	no
Product	minor citrate synthase
Function	unknown
Gene expression levels in SubtiExpress: citA
Metabolic function and regulation of this protein in SubtiPathways: citA
MW, pI	40 kDa, 5.778
Gene length, protein length	1098 bp, 366 aa
Immediate neighbours	citR, yhdF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

poorly characterized/ putative enzymes, sporulation proteins

This gene is a member of the following regulons

CitR regulon

The gene

Basic information

• Locus tag: BSU09440

Database entries

• BsubCyc: BSU09440

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acetyl-CoA + H₂O + oxaloacetate = citrate + CoA (according to Swiss-Prot)

• Protein family: citrate synthase family (according to Swiss-Prot)

• Paralogous protein(s): MmgD, CitZ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU09440

• Structure: 2C6X

• UniProt: P39119

• KEGG entry: [3]

• E.C. number: 2.3.3.1

Expression and regulation

• Operon: citA-yhdF PubMed

• Expression browser: citA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism: CitR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • GP676 (erm), available in the Stülke lab
  • GP1282 (citA::cat), available in Jörg Stülke's lab
  • GP1753 (citR citA::aphA3), the kanamycin resistance cassette can be cut out by introducing the cre-rekombinase into the chromosom of B. subtilis, available in Jörg Stülke's lab

• Expression vector:
  • pGP2516 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• FLAG-tag construct:
  • GP1287 (spc, based on pGP1331), available in Jörg Stülke's lab

• Antibody: