Difference between revisions of "CitA"
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** GP676 (erm), available in the [[Stülke]] lab | ** GP676 (erm), available in the [[Stülke]] lab | ||
** GP1282 (''[[citA]]''::''cat''), available in [[Jörg Stülke]]'s lab | ** GP1282 (''[[citA]]''::''cat''), available in [[Jörg Stülke]]'s lab | ||
| − | ** GP1753 (''citR'' ''citA''::''aphA3''), available in [[Jörg Stülke]]'s lab | + | ** GP1753 (''[[citR'']] ''citA''::''aphA3''), available in [[Jörg Stülke]]'s lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
Revision as of 14:05, 8 December 2014
- Description: minor citrate synthase
| Gene name | citA |
| Synonyms | citA1 |
| Essential | no |
| Product | minor citrate synthase |
| Function | unknown |
| Gene expression levels in SubtiExpress: citA | |
| Metabolic function and regulation of this protein in SubtiPathways: citA | |
| MW, pI | 40 kDa, 5.778 |
| Gene length, protein length | 1098 bp, 366 aa |
| Immediate neighbours | citR, yhdF |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
poorly characterized/ putative enzymes, sporulation proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU09440
Phenotypes of a mutant
Database entries
- BsubCyc: BSU09440
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Acetyl-CoA + H2O + oxaloacetate = citrate + CoA (according to Swiss-Prot)
- Protein family: citrate synthase family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU09440
- Structure: 2C6X
- UniProt: P39119
- KEGG entry: [3]
- E.C. number: 2.3.3.1
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- GP676 (erm), available in the Stülke lab
- GP1282 (citA::cat), available in Jörg Stülke's lab
- GP1753 (citR'' citA::aphA3), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- FLAG-tag construct:
- GP1287 (spc, based on pGP1331), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849]
[WorldCat.org]
[DOI]
(I p)
S Jin, A L Sonenshein
Transcriptional regulation of Bacillus subtilis citrate synthase genes.
J Bacteriol: 1994, 176(15);4680-90
[PubMed:8045899]
[WorldCat.org]
[DOI]
(P p)
S Jin, A L Sonenshein
Identification of two distinct Bacillus subtilis citrate synthase genes.
J Bacteriol: 1994, 176(15);4669-79
[PubMed:8045898]
[WorldCat.org]
[DOI]
(P p)
