EzrA

• Description: negative regulator of FtsZ ring formation
  
  

Gene name	ezrA
Synonyms	ytwP
Essential	no
Product	FtsZ-interacting protein
Function	control of FtsZ ring formation
Gene expression levels in SubtiExpress: ezrA
Interactions involving this protein in SubtInteract: EzrA
MW, pI	64 kDa, 4.757
Gene length, protein length	1686 bp, 562 aa
Immediate neighbours	braB, hisJ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell division, membrane proteins

The gene

Basic information

• Locus tag: BSU29610

Phenotypes of a mutant

• altered localization of FtsZ and PBP1 PubMed
• growth defects, elongated cells PubMed
• the ezrA mutation is synthetically lethal with a sepF mutation PubMed
• a yvcL ezrA double mutant grows poorly, and the cells are filamentous PubMed
• a zapA ezrA double mutant forms filamentous cells PubMed
• depletion of pdhA and deletion of ezrA have a strong synthetic defect in cell division PubMed

Database entries

• BsubCyc: BSU29610

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • inhibits FtsZ polymerization PubMed
  • recruits PBP1 to the division septum PubMed
  • activates PrkC PubMed

• Protein family: ezrA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:
  • degradation of EzrA involves FtsH PubMed

• Interactions:
  • EzrA-UgtP PubMed
  • FtsZ-EzrA PubMed
  • EzrA-GpsB PubMed
  • EzrA-PonA PubMed

• Localization:
  • membrane associated PubMed
  • 1 transmembrane domain at the N-terminus PubMed

Database entries

• BsubCyc: BSU29610

• Structure: 4UXV (the 60 kDa cytoplasmic domain) PubMed

• UniProt: O34894

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ezrA PubMed

• Expression browser: ezrA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • constitutively expressed PubMed

• Regulatory mechanism:

• Additional information:
  • degradation of EzrA involves FtsH PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 125 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 495 PubMed

Biological materials

• Mutant:
  • 1A805 ( ezrA::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Marc Bramkamp, Suey van Baarle
Division site selection in rod-shaped bacteria.
Curr Opin Microbiol: 2009, 12(6);683-8
[PubMed:19884039] [WorldCat.org] [DOI] (I p)

David W Adams, Jeff Errington
Bacterial cell division: assembly, maintenance and disassembly of the Z ring.
Nat Rev Microbiol: 2009, 7(9);642-53
[PubMed:19680248] [WorldCat.org] [DOI] (I p)