PerR

• Description: transcriptional repressor of the peroxide regulon, sensor of the intracellular Fe/Mn ratio
  
  

Gene name	perR
Synonyms	ygaG
Essential	no
Product	transcriptional repressor (Fur family)
Function	regulation of the response to peroxide
Gene expression levels in SubtiExpress: perR
Metabolic function and regulation of this protein in SubtiPathways: perR
MW, pI	16 kDa, 5.888
Gene length, protein length	435 bp, 145 aa
Immediate neighbours	ygaF, ygzB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

PerR regulon

The gene

Basic information

• Locus tag: BSU08730

Phenotypes of a mutant

• resistant to hydrogen peroxide, accumulates a porphyrin-like compound, and grows very slowly (due to heme sequestration by KatA) PubMed

Database entries

• BsubCyc: BSU08730

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: Fur family

• Paralogous protein(s): Zur, Fur

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • selective metal catalyzed oxidation of two histidine residues of the regulatory site results in induction (loss of DNA-binding activity) PubMed

• Cofactor(s): contains an Fe(2+) at the regulatory site and Zn(2+) PubMed

• Effectors of protein activity:
  • responds to the presence of hydrogen peroxide

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU08730

• Structure: 2RGV 3F8N

• UniProt: P71086

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: perR PubMed

• Expression browser: perR PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • negative autoregulation PubMed
  • induced in the presence of hydrogen peroxide (PerR) PubMed

• Regulatory mechanism:
  • PerR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: HB0509 (spc), available in John Helmann's and Jörg Stülke's labs, also GP868 (fur::mls, perR::spc).
  • 1A903 ( perR::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

References

Reviews

John D Helmann
Specificity of metal sensing: iron and manganese homeostasis in Bacillus subtilis.
J Biol Chem: 2014, 289(41);28112-20
[PubMed:25160631] [WorldCat.org] [DOI] (I p)

James M Dubbs, Skorn Mongkolsuk
Peroxide-sensing transcriptional regulators in bacteria.
J Bacteriol: 2012, 194(20);5495-503
[PubMed:22797754] [WorldCat.org] [DOI] (I p)

Melinda J Faulkner, John D Helmann
Peroxide stress elicits adaptive changes in bacterial metal ion homeostasis.
Antioxid Redox Signal: 2011, 15(1);175-89
[PubMed:20977351] [WorldCat.org] [DOI] (I p)

Victor Duarte, Jean-Marc Latour
PerR vs OhrR: selective peroxide sensing in Bacillus subtilis.
Mol Biosyst: 2010, 6(2);316-23
[PubMed:20094649] [WorldCat.org] [DOI] (I p)

Peter Zuber
Management of oxidative stress in Bacillus.
Annu Rev Microbiol: 2009, 63;575-97
[PubMed:19575568] [WorldCat.org] [DOI] (I p)

David P Giedroc
Hydrogen peroxide sensing in Bacillus subtilis: it is all about the (metallo)regulator.
Mol Microbiol: 2009, 73(1);1-4
[PubMed:19508286] [WorldCat.org] [DOI] (I p)