Difference between revisions of "Scr"
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* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU_MISC_RNA_2&redirect=T BSU_misc_RNA_2] | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU_MISC_RNA_2&redirect=T BSU_misc_RNA_2] | ||
| − | * '''Structure''': | + | * '''Structure''': [http://www.rcsb.org/pdb/explore.do?structureId=4WFL 4WFL] {{PubMed|25270875}} |
=== Additional information=== | === Additional information=== | ||
| Line 112: | Line 112: | ||
<pubmed> 25212246 </pubmed> | <pubmed> 25212246 </pubmed> | ||
== Original publications == | == Original publications == | ||
| − | <pubmed>9346318,2468993,17576666, 1372600,7506707 1383945 7538759 10224127 9677377 8662730 10222262</pubmed> | + | <pubmed>9346318,2468993,17576666, 1372600,7506707 1383945 7538759 10224127 9677377 8662730 10222262 25270875 </pubmed> |
[[Category:small RNA]] | [[Category:small RNA]] | ||
Revision as of 15:22, 4 October 2014
- Description: 4.5 S RNA
| Gene name | scr |
| Synonyms | |
| Essential | yes |
| Product | small cytoplasmic RNA (scRNA, 4.5S RNA),
signal recognition particle-like (SRP) component |
| Function | presecretory protein translocation |
| Interactions involving this RNA in SubtInteract: Scr | |
| Immediate neighbours | yaaJ, dnaX |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
protein secretion, essential genes, ncRNA
This gene is a member of the following regulons
The gene
Basic information
- Locus tag:
Phenotypes of a mutant
- the gene is essential PubMed
Database entries
- BsubCyc: BSU_misc_RNA_2
- DBTBS entry: no entry
- SubtiList entry: [1]
- Structure:
Additional information
The RNA
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
Extended information on the RNA
- Kinetic information
Database entries
- BsubCyc: BSU_misc_RNA_2
Additional information
Expression and regulation
- Sigma factor: SigA (both promoters)
- Regulation:
- Regulatory mechanism:
- Additional information: transcribed as 354 nt precursor, cleaved at the 5' end by RNase III and at the 3' site by RNase J1 to yield a 275 nt molecule, final trimming to 271 nt sc RNA by RNase PH PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
Labs working on this gene/protein
David Bechhofer, Mount Sinai School, New York, USA Homepage
Your additional remarks
References
Reviews
Zhen Kang, Sen Yang, Guocheng Du, Jian Chen
Molecular engineering of secretory machinery components for high-level secretion of proteins in Bacillus species.
J Ind Microbiol Biotechnol: 2014, 41(11);1599-607
[PubMed:25212246]
[WorldCat.org]
[DOI]
(I p)
Original publications
Georg Kempf, Klemens Wild, Irmgard Sinning
Structure of the complete bacterial SRP Alu domain.
Nucleic Acids Res: 2014, 42(19);12284-94
[PubMed:25270875]
[WorldCat.org]
[DOI]
(I p)
Shiyi Yao, Joshua B Blaustein, David H Bechhofer
Processing of Bacillus subtilis small cytoplasmic RNA: evidence for an additional endonuclease cleavage site.
Nucleic Acids Res: 2007, 35(13);4464-73
[PubMed:17576666]
[WorldCat.org]
[DOI]
(I p)
K Nakamura, S Yahagi, T Yamazaki, K Yamane
Bacillus subtilis histone-like protein, HBsu, is an integral component of a SRP-like particle that can bind the Alu domain of small cytoplasmic RNA.
J Biol Chem: 1999, 274(19);13569-76
[PubMed:10224127]
[WorldCat.org]
[DOI]
(P p)
T Yamazaki, S Yahagi, K Nakamura, K Yamane
Depletion of Bacillus subtilis histone-like protein, HBsu, causes defective protein translocation and induces upregulation of small cytoplasmic RNA.
Biochem Biophys Res Commun: 1999, 258(1);211-4
[PubMed:10222262]
[WorldCat.org]
[DOI]
(P p)
A Oguro, H Kakeshita, K Nakamura, K Yamane, W Wang, D H Bechhofer
Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor.
J Biol Chem: 1998, 273(31);19542-7
[PubMed:9677377]
[WorldCat.org]
[DOI]
(P p)
H Takamatsu, K Bunai, T Horinaka, A Oguro, K Nakamura, K Watabe, K Yamane
Identification of a region required for binding to presecretory protein in Bacillus subtilis Ffh, a homologue of the 54-kDa subunit of mammalian signal recognition particle.
Eur J Biochem: 1997, 248(2);575-82
[PubMed:9346318]
[WorldCat.org]
[DOI]
(P p)
K Kurita, K Honda, S Suzuma, H Takamatsu, K Nakamura, K Yamane
Identification of a region of Bacillus subtilis Ffh, a homologue of mammalian SRP54 protein, that is essential for binding to small cytoplasmic RNA.
J Biol Chem: 1996, 271(22);13140-6
[PubMed:8662730]
[WorldCat.org]
[DOI]
(P p)
T Shibata, Y Nakamura, K Nakamura, K Yamane
Depletion of small cytoplasmic RNA confers fusidic-acid resistance on Bacillus subtilis.
Biochem Biophys Res Commun: 1995, 210(2);317-23
[PubMed:7538759]
[WorldCat.org]
[DOI]
(P p)
M Nishiguchi, K Honda, R Amikura, K Nakamura, K Yamane
Structural requirements of Bacillus subtilis small cytoplasmic RNA for cell growth, sporulation, and extracellular enzyme production.
J Bacteriol: 1994, 176(1);157-65
[PubMed:7506707]
[WorldCat.org]
[DOI]
(P p)
K Nakamura, M Minemura, M Nishiguchi, K Honda, A Nakamura, K Yamane
Conserved residues and secondary structure found in small cytoplasmic RNAs from thirteen Bacillus species.
Nucleic Acids Res: 1992, 20(19);5227-8
[PubMed:1383945]
[WorldCat.org]
[DOI]
(P p)
K Nakamura, Y Imai, A Nakamura, K Yamane
Small cytoplasmic RNA of Bacillus subtilis: functional relationship with human signal recognition particle 7S RNA and Escherichia coli 4.5S RNA.
J Bacteriol: 1992, 174(7);2185-92
[PubMed:1372600]
[WorldCat.org]
[DOI]
(P p)
J C Struck, R K Hartmann, H Y Toschka, V A Erdmann
Transcription and processing of Bacillus subtilis small cytoplasmic RNA.
Mol Gen Genet: 1989, 215(3);478-82
[PubMed:2468993]
[WorldCat.org]
[DOI]
(P p)
