SigA

• Description: RNA polymerase major sigma factor SigA
  
  

Gene name	sigA
Synonyms	rpoD, crsA
Essential	yes PubMed
Product	RNA polymerase major sigma factor SigA
Function	transcription
Gene expression levels in SubtiExpress: sigA
Interactions involving this protein in SubtInteract: SigA
MW, pI	42 kDa, 4.634
Gene length, protein length	1113 bp, 371 aa
Immediate neighbours	cccA, dnaG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, sigma factors and their control, essential genes, membrane proteins

This gene is a member of the following regulons

SigD regulon, SigH regulon

The gene

Basic information

• Locus tag: BSU25200

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU25200

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• List of SigA controlled genes

• Protein family: sigma-70 factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • Spo0A-SigA PubMed
  • SigA-(RpoB-RpoC) PubMed
  • BsrA-SigA-RNA polymerase
  • BsrB-SigA-RNA polymerase

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU25200

• Structure:

• UniProt: P06224

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • yqxD-dnaG-sigA PubMed
  • sigA PubMed

• Expression browser: sigA PubMed

• Sigma factor:
  • yqxD: SigA PubMed, SigH PubMed
  • sigA: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • An antisense RNA is predicted for sigA PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 802 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1811 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1136 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 2062 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1407 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Charles Moran, Emory University, NC, USA homepage

References

Reviews

Benedikt Steuten, Sabine Schneider, Rolf Wagner
6S RNA: recent answers--future questions.
Mol Microbiol: 2014, 91(4);641-8
[PubMed:24308327] [WorldCat.org] [DOI] (I p)

Lakshminarayan M Iyer, L Aravind
Insights from the architecture of the bacterial transcription apparatus.
J Struct Biol: 2012, 179(3);299-319
[PubMed:22210308] [WorldCat.org] [DOI] (I p)