Rtp

• Description: replication terminator protein
  
  

Gene name	rtp
Synonyms
Essential	no
Product	replication terminator protein
Function	replication termination
Gene expression levels in SubtiExpress: rtp
MW, pI	14 kDa, 9.778
Gene length, protein length	366 bp, 122 aa
Immediate neighbours	proH, yoxD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication

The gene

Basic information

• Locus tag: BSU18490

Database entries

• BsubCyc: BSU18490

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binds to nine termination sites spanning ∼430 kb of the replication terminus region PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU18490

• Structure: 1F4K (complex with DNA), 1BM9

• UniProt: P68732

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: rtp PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 50 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Daniel L Kaplan, Deepak Bastia
Mechanisms of polar arrest of a replication fork.
Mol Microbiol: 2009, 72(2);279-85
[PubMed:19298368] [WorldCat.org] [DOI] (I p)

Original publications

Qiaojuan Shi, Jose C Huguet-Tapia, Joseph E Peters
Tn917 targets the region where DNA replication terminates in Bacillus subtilis, highlighting a difference in chromosome processing in the firmicutes.
J Bacteriol: 2009, 191(24);7623-7
[PubMed:19820088] [WorldCat.org] [DOI] (I p)

Iain G Duggin, Jacqueline M Matthews, Nicholas E Dixon, R Gerry Wake, Joel P Mackay
A complex mechanism determines polarity of DNA replication fork arrest by the replication terminator complex of Bacillus subtilis.
J Biol Chem: 2005, 280(13);13105-13
[PubMed:15657033] [WorldCat.org] [DOI] (P p)

A Gautam, S Mulugu, K Alexander, D Bastia
A single domain of the replication termination protein of Bacillus subtilis is involved in arresting both DnaB helicase and RNA polymerase.
J Biol Chem: 2001, 276(26);23471-9
[PubMed:11313334] [WorldCat.org] [DOI] (P p)

P A Andersen, A A Griffiths, I G Duggin, R G Wake
Functional specificity of the replication fork-arrest complexes of Bacillus subtilis and Escherichia coli: significant specificity for Tus-Ter functioning in E. coli.
Mol Microbiol: 2000, 36(6);1327-35
[PubMed:10931283] [WorldCat.org] [DOI] (P p)

S Autret, A Levine, F Vannier, Y Fujita, S J Séror
The replication checkpoint control in Bacillus subtilis: identification of a novel RTP-binding sequence essential for the replication fork arrest after induction of the stringent response.
Mol Microbiol: 1999, 31(6);1665-79
[PubMed:10209741] [WorldCat.org] [DOI] (P p)

I G Duggin, P A Andersen, M T Smith, J A Wilce, G F King, R G Wake
Site-directed mutants of RTP of Bacillus subtilis and the mechanism of replication fork arrest.
J Mol Biol: 1999, 286(5);1325-35
[PubMed:10064700] [WorldCat.org] [DOI] (P p)

A A Griffiths, P A Andersen, R G Wake
Replication terminator protein-based replication fork-arrest systems in various Bacillus species.
J Bacteriol: 1998, 180(13);3360-7
[PubMed:9642188] [WorldCat.org] [DOI] (P p)

M T Smith, C J de Vries, D B Langley, G F King, R G Wake
The Bacillus subtilis DNA replication terminator.
J Mol Biol: 1996, 260(1);54-69
[PubMed:8676392] [WorldCat.org] [DOI] (P p)

A H Franks, A A Griffiths, R G Wake
Identification and characterization of new DNA replication terminators in Bacillus subtilis.
Mol Microbiol: 1995, 17(1);13-23
[PubMed:7476199] [WorldCat.org] [DOI] (P p)

A Levine, S Autret, S J Séror
A checkpoint involving RTP, the replication terminator protein, arrests replication downstream of the origin during the Stringent Response in Bacillus subtilis.
Mol Microbiol: 1995, 15(2);287-95
[PubMed:7746150] [WorldCat.org] [DOI] (P p)

D B Langley, M T Smith, P J Lewis, R G Wake
Protein-nucleoside contacts in the interaction between the replication terminator protein of Bacillus subtilis and the DNA terminator.
Mol Microbiol: 1993, 10(4);771-9
[PubMed:7934839] [WorldCat.org] [DOI] (P p)

P J Lewis, R G Wake
Termination of chromosome replication in Bacillus subtilis.
Res Microbiol: 1991, 142(7-8);893-900
[PubMed:1784827] [WorldCat.org] [DOI] (P p)

P J Lewis, G B Ralston, R I Christopherson, R G Wake
Identification of the replication terminator protein binding sites in the terminus region of the Bacillus subtilis chromosome and stoichiometry of the binding.
J Mol Biol: 1990, 214(1);73-84
[PubMed:2115089] [WorldCat.org] [DOI] (P p)

P J Lewis, M T Smith, R G Wake
A protein involved in termination of chromosome replication in Bacillus subtilis binds specifically to the terC site.
J Bacteriol: 1989, 171(6);3564-7
[PubMed:2498294] [WorldCat.org] [DOI] (P p)