ComQ

• Description: prenylation of ComX, regulator of late competence operon (comG) and surfactin expression (srfA)
  
  

Gene name	comQ
Synonyms
Essential	no
Product	prenyltransferase
Function	regulation of quorum sensing
Gene expression levels in SubtiExpress: comQ
Interactions involving this protein in SubtInteract: ComQ
MW, pI	34 kDa, 4.893
Gene length, protein length	897 bp, 299 aa
Immediate neighbours	comX, degQ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, quorum sensing, membrane proteins

This gene is a member of the following regulons

PhoP regulon

The gene

Basic information

• Locus tag: BSU31710

Phenotypes of a mutant

• the mutation suppresses the mucoid phenotype of motA or motB mutants due to loss of DegU phosphorylation and concomitant reduced expression of the capB-capC-capA-capE operon PubMed

Database entries

• BsubCyc: BSU31710

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • prenylation of ComX PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • ComQ-ComX PubMed

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU31710

• Structure:

• UniProt: P33690

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: comQ-comX PubMed

• Expression browser: comQ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Letal I Salzberg, Eric Botella, Karsten Hokamp, Haike Antelmann, Sandra Maaß, Dörte Becher, David Noone, Kevin M Devine
Genome-wide analysis of phosphorylated PhoP binding to chromosomal DNA reveals several novel features of the PhoPR-mediated phosphate limitation response in Bacillus subtilis.
J Bacteriol: 2015, 197(8);1492-506
[PubMed:25666134] [WorldCat.org] [DOI] (I p)

Masahiro Okada, Ayako Ishihara, Risa Yamasaki, Fumitada Tsuji, Shunsuke Hayashi, Syohei Usami, Youji Sakagami
A region corresponding to second aspartate-rich motif in tryptophan isoprenylating enzyme, ComQ, serves as a substrate-binding site.
Biosci Biotechnol Biochem: 2014, 78(4);550-5
[PubMed:25036949] [WorldCat.org] [DOI] (I p)

Iztok Dogsa, Kumari Sonal Choudhary, Ziva Marsetic, Sanjarbek Hudaiberdiev, Roberto Vera, Sándor Pongor, Ines Mandic-Mulec
ComQXPA quorum sensing systems may not be unique to Bacillus subtilis: a census in prokaryotic genomes.
PLoS One: 2014, 9(5);e96122
[PubMed:24788106] [WorldCat.org] [DOI] (I e)

Anna Oslizlo, Polonca Stefanic, Iztok Dogsa, Ines Mandic-Mulec
Private link between signal and response in Bacillus subtilis quorum sensing.
Proc Natl Acad Sci U S A: 2014, 111(4);1586-91
[PubMed:24425772] [WorldCat.org] [DOI] (I p)

Jia Mun Chan, Sarah B Guttenplan, Daniel B Kearns
Defects in the flagellar motor increase synthesis of poly-γ-glutamate in Bacillus subtilis.
J Bacteriol: 2014, 196(4);740-53
[PubMed:24296669] [WorldCat.org] [DOI] (I p)

Fumitada Tsuji, Ayako Ishihara, Aya Nakagawa, Masahiro Okada, Shigeyuki Kitamura, Kyoko Kanamaru, Yuichi Masuda, Kazuma Murakami, Kazuhiro Irie, Youji Sakagami
Lack of the consensus sequence necessary for tryptophan prenylation in the ComX pheromone precursor.
Biosci Biotechnol Biochem: 2012, 76(8);1492-6
[PubMed:22878193] [WorldCat.org] [DOI] (I p)

Daniel López, Hera Vlamakis, Richard Losick, Roberto Kolter
Paracrine signaling in a bacterium.
Genes Dev: 2009, 23(14);1631-8
[PubMed:19605685] [WorldCat.org] [DOI] (I p)

Polonca Stefanic, Ines Mandic-Mulec
Social interactions and distribution of Bacillus subtilis pherotypes at microscale.
J Bacteriol: 2009, 191(6);1756-64
[PubMed:19114482] [WorldCat.org] [DOI] (I p)

Mireille Ansaldi, Darja Marolt, Tina Stebe, Ines Mandic-Mulec, David Dubnau
Specific activation of the Bacillus quorum-sensing systems by isoprenylated pheromone variants.
Mol Microbiol: 2002, 44(6);1561-73
[PubMed:12067344] [WorldCat.org] [DOI] (P p)

P Tortosa, L Logsdon, B Kraigher, Y Itoh, I Mandic-Mulec, D Dubnau
Specificity and genetic polymorphism of the Bacillus competence quorum-sensing system.
J Bacteriol: 2001, 183(2);451-60
[PubMed:11133937] [WorldCat.org] [DOI] (P p)

Y Weinrauch, T Msadek, F Kunst, D Dubnau
Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis.
J Bacteriol: 1991, 173(18);5685-93
[PubMed:1715859] [WorldCat.org] [DOI] (P p)