prpC

168

protein phosphatase

Locus: BSU_15760

Molecular weight: 27.52 kDa

Isoelectric point: 4.36

Protein length: 254 aa

Gene length: 765 bp

Function: antagonist of PrkC-dependent phosphorylation

Product: protein phosphatase

Essential: no

E.C.: 3.1.3.16

Synonyms: prpC, yloO

Genomic Context

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List of homologs in different organisms, belongs to COG0631 (Galperin et al., 2021)

Gene

Coordinates: 1,650,384  1,651,148

Phenotypes of a mutant

• A prpC mutant is less lytic in late stationary phase. PubMed
• inactivation of ''prpC'' reduces sporulation efficiency to 50.7% that of wild type cells PubMed

The protein

Catalyzed reaction/ biological activity

• dephosphorylation of EF-tu]]-Thr63-P PubMed
• H₂O + O-phospho-L-seryl-[protein] --> L-seryl-[protein] + phosphate (according to UniProt)
• H₂O + O-phospho-L-threonyl-[protein] --> L-threonyl-[protein] + phosphate (according to UniProt)

Domains

• PPM-type phosphatase domain (aa 2-243) (according to UniProt)

Cofactors

• divalent cations such as magnesium or manganese

Structure

• 1TXO (PDB) (from Mycobacterium tuberculosis, 34% identity, 54% similarity) PubMed
• O34779 (AlphaFold)

Effectors of protein activity

• inhibited by inorganic phosphate and glycero-2-phosphate PubMed

Expression and Regulation

Operons

• Genes: prpC-prkC-cpgA-rpeA-yloS

  Description: PubMed

Biological materials

Mutant

• MGNA-B133 (yloO::erm), available at the NBRP B. subtilis, Japan
• OMG401 (aphA3), available in Jörg Stülke's lab
• 1A962 (no resistance), PubMed, available at BGSC
• 1A964 (no resistance), PubMed, available at BGSC
• BKE15760 (prpC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTAAGGCTGTTAACAACC,  downstream forward: _UP4_CAAGTTGAAGAGGGTGAAGA
• BKK15760 (prpC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTAAGGCTGTTAACAACC,  downstream forward: _UP4_CAAGTTGAAGAGGGTGAAGA

References

Reviews

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Original Publications

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