RuvA

• Description: Holliday junction DNA helicase, part of the RuvA-RuvB branch migration translocase
  
  

Gene name	ruvA
Synonyms
Essential	no
Product	Holliday junction DNA helicase
Function	DNA repair/ recombination
Gene expression levels in SubtiExpress: ruvA
Interactions involving this protein in SubtInteract: RuvA
MW, pI	22 kDa, 5.384
Gene length, protein length	603 bp, 201 aa
Immediate neighbours	ruvB, bofC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU27740

Phenotypes of a mutant

• the inactivations of recU and ruvA-ruvB are synthetically lethal PubMed

Database entries

• BsubCyc: BSU27740

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • inhibits resolution of Holliday junctions by RecU-RuvB PubMed

• Protein family: ruvA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • RuvA-RuvB

• Localization:

Database entries

• BsubCyc: BSU27740

• Structure:

• UniProt: O05392

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ruvA-ruvB PubMed

• Expression browser: ruvA PubMed

• Sigma factor:

• Regulation:
  • induced upon DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A896 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

S C West
Processing of recombination intermediates by the RuvABC proteins.
Annu Rev Genet: 1997, 31;213-44
[PubMed:9442895] [WorldCat.org] [DOI] (P p)