Difference between revisions of "YwrD"

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(Basic information/ Evolution)
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* '''Description:''' unknown <br/><br/>
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* '''Description:''' unknown, similar to gamma-glutamyltransferase ([[Ggt]])<br/><br/>
  
 
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Revision as of 08:27, 5 May 2014

  • Description: unknown, similar to gamma-glutamyltransferase (Ggt)

Gene name ywrD
Synonyms
Essential no
Product unknown
Function unknown
Gene expression levels in SubtiExpress: ywrD
MW, pI 57 kDa, 5.451
Gene length, protein length 1575 bp, 525 aa
Immediate neighbours ywrE, chrS
Sequences Protein DNA DNA_with_flanks
Genetic context
YwrD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YwrD expression.png
























Categories containing this gene/protein

proteins of unknown function

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

  • Locus tag: BSU36100

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl amino acid (according to Swiss-Prot), however, all evidence suggests this is a misannotation PubMed
  • Protein family: gamma-glutamyltransferase family (according to Swiss-Prot), however, all evidence suggests this is a misannotation PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

The gene is annotated in KEGG as an ortholog of gamma-glutamyltranspeptidase EC 2.3.2.2, This enzyme is necessary to utilize glutathione (GSH) as the sulfur source. No EC annotation for this gene is available in Swiss-Prot/MetaCyc. It has been shown by Minami et al. (PubMed) that ywrD mutant grows well on minimal media supplied with GSH as the sole sulfur source. In addition, His-tag purified ywrD cannot hydrolyze GSH. PubMed


Expression and regulation

  • Sigma factor:
  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References