Difference between revisions of "YvcN"
(→Biological materials) |
|||
Line 133: | Line 133: | ||
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
− | + | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab | |
− | * '''two-hybrid system:''' | ||
* '''Antibody:''' | * '''Antibody:''' |
Revision as of 13:24, 8 December 2014
- Description: unknown
Gene name | yvcN |
Synonyms | |
Essential | no |
Product | unknown |
Function | unknown |
Gene expression levels in SubtiExpress: yvcN | |
MW, pI | 28 kDa, 5.582 |
Gene length, protein length | 762 bp, 254 aa |
Immediate neighbours | yvzJ, crh |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34730
Phenotypes of a mutant
Database entries
- BsubCyc: BSU34730
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: arylamine N-acetyltransferase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU34730
- Structure:
- UniProt: O06977
- KEGG entry: [2]
- E.C. number:
Additional information
The gene is annotated in KEGG as an ortholog of EC 2.3.1.5 and is marked in Swiss-Prot as “uncharacterized acetyltransferase” (EC 2.3.1.-). No EC annotation is available in MetaCyc.No literature/ experimental evidence supporting the annotation is available. PubMed
Expression and regulation
- Regulation: very weak stimuation of expression by citrate and succinate PubMed
- Regulatory mechanism:
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium): 84 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 363 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 290 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 680 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Boris Görke, Elodie Foulquier, Anne Galinier
YvcK of Bacillus subtilis is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway.
Microbiology (Reading): 2005, 151(Pt 11);3777-3791
[PubMed:16272399]
[WorldCat.org]
[DOI]
(P p)
A Galinier, J Haiech, M C Kilhoffer, M Jaquinod, J Stülke, J Deutscher, I Martin-Verstraete
The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression.
Proc Natl Acad Sci U S A: 1997, 94(16);8439-44
[PubMed:9237995]
[WorldCat.org]
[DOI]
(P p)