Difference between revisions of "YvcI"
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* '''GFP fusion:''' | * '''GFP fusion:''' | ||
− | * '''two-hybrid system:''' | + | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab |
* '''Antibody:''' | * '''Antibody:''' |
Revision as of 13:23, 8 December 2014
- Description: Nudix hydrolase
Gene name | yvcI |
Synonyms | |
Essential | no |
Product | unknown |
Function | unknown |
Gene expression levels in SubtiExpress: yvcI | |
MW, pI | 18 kDa, 4.993 |
Gene length, protein length | 474 bp, 158 aa |
Immediate neighbours | yvcJ, trxB |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34780
Phenotypes of a mutant
Database entries
- BsubCyc: BSU34780
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: Nudix hydrolase
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU34780
- Structure:
- UniProt: O06972
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation: repressed by glucose (4.5-fold) (CcpA) PubMed, very weak stimuation of expression by citrate and succinate PubMed
- Regulatory mechanism: CcpA: transcription repression
- Additional information:
- A ncRNA is predicted between yvcI and trxB PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 67 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 601 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 472 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 816 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References