Difference between revisions of "MgtE"
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=References= | =References= | ||
− | <pubmed>17803910,15096624, 21315082, 22970223 24415722</pubmed> | + | <pubmed>17803910,15096624, 21315082, 22970223 24415722 25182490 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 13:21, 6 September 2014
- Description: primary magnesium transporter
Gene name | mgtE |
Synonyms | ykoK |
Essential | no |
Product | primary magnesium transporter |
Function | magnesium uptake |
Gene expression levels in SubtiExpress: mgtE | |
Metabolic function and regulation of this protein in SubtiPathways: MgtE | |
MW, pI | 50 kDa, 4.609 |
Gene length, protein length | 1353 bp, 451 aa |
Immediate neighbours | ykzO, tnrA |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13300
Phenotypes of a mutant
- a mgtE mutant has a growth defect on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medim in the presence of citrate PubMed
- a mgtE yfjQ mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medim in the presence of citrate PubMed
Database entries
- BsubCyc: BSU13300
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: SLC41A transporter family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- BsubCyc: BSU13300
- Structure: 2QBZ (the mgtE riboswitch)
- UniProt: O34442
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism: M-box: Mg(2+)-responsive riboswitch PubMed
- Additional information:
- number of protein molecules per cell (complex medium with amino acids, without glucose): 259 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Genki Akanuma, Ako Kobayashi, Shota Suzuki, Fujio Kawamura, Yuh Shiwa, Satoru Watanabe, Hirofumi Yoshikawa, Ryo Hanai, Morio Ishizuka
Defect in the formation of 70S ribosomes caused by lack of ribosomal protein L34 can be suppressed by magnesium.
J Bacteriol: 2014, 196(22);3820-30
[PubMed:25182490]
[WorldCat.org]
[DOI]
(I p)
Catherine A Wakeman, Jonathan R Goodson, Vineetha M Zacharia, Wade C Winkler
Assessment of the requirements for magnesium transporters in Bacillus subtilis.
J Bacteriol: 2014, 196(6);1206-14
[PubMed:24415722]
[WorldCat.org]
[DOI]
(I p)
Jaya Sahni, Yumei Song, Andrew M Scharenberg
The B. subtilis MgtE magnesium transporter can functionally compensate TRPM7-deficiency in vertebrate B-cells.
PLoS One: 2012, 7(9);e44452
[PubMed:22970223]
[WorldCat.org]
[DOI]
(I p)
Arati Ramesh, Catherine A Wakeman, Wade C Winkler
Insights into metalloregulation by M-box riboswitch RNAs via structural analysis of manganese-bound complexes.
J Mol Biol: 2011, 407(4);556-70
[PubMed:21315082]
[WorldCat.org]
[DOI]
(I p)
Charles E Dann, Catherine A Wakeman, Cecelia L Sieling, Stephanie C Baker, Irnov Irnov, Wade C Winkler
Structure and mechanism of a metal-sensing regulatory RNA.
Cell: 2007, 130(5);878-92
[PubMed:17803910]
[WorldCat.org]
[DOI]
(P p)
Jeffrey E Barrick, Keith A Corbino, Wade C Winkler, Ali Nahvi, Maumita Mandal, Jennifer Collins, Mark Lee, Adam Roth, Narasimhan Sudarsan, Inbal Jona, J Kenneth Wickiser, Ronald R Breaker
New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control.
Proc Natl Acad Sci U S A: 2004, 101(17);6421-6
[PubMed:15096624]
[WorldCat.org]
[DOI]
(P p)