NfrA

• Description: Spx-dependent FMN-containing NADPH-linked nitro/flavin reductase, stress protein
  
  

Gene name	nfrA
Synonyms	ywcG, ipa-43d
Essential	no
Product	FMN-containing NADPH-linked nitro/flavin reductase
Function	unknown
Gene expression levels in SubtiExpress: nfrA
Metabolic function and regulation of this protein in SubtiPathways: nfrA
MW, pI	28 kDa, 5.732
Gene length, protein length	747 bp, 249 aa
Immediate neighbours	ywcH, rodA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

electron transport/ other, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

SigD regulon, Spo0A regulon, Spx regulon

The gene

Basic information

• Locus tag: BSU38110

Database entries

• BsubCyc: BSU38110

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD(+) degrading activity leading to free nicotinamide PubMed

• Protein family: flavin oxidoreductase frp family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU38110

• Structure: 3N2S PubMed

• UniProt: P39605

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nfrA-ywcH PubMed

• Expression browser: nfrA PubMed

• Sigma factor: SigA PubMed, SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • Spx: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 222 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 616 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 560 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 960 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: