MotA

• Description: H+-coupled MotA-MotB flagellar stator
  
  

Gene name	motA
Synonyms	mot
Essential	no
Product	motility protein (flagellar motor rotation)
Function	motility and chemotaxis
Gene expression levels in SubtiExpress: motA
Interactions involving this protein in SubtInteract: MotA
MW, pI	29 kDa, 5.01
Gene length, protein length	810 bp, 270 aa
Immediate neighbours	motB, clpE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

• Locus tag: BSU13690

Phenotypes of a mutant

• loss of swimming and swarming motility PubMed
• mucoid phenotype due to the DegU-P activated overexpression of the capB-capC-capA-capE operon and resulting overproduction of poly-gamma-glutamate PubMed

Database entries

• BsubCyc: BSU13690

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: motA family (according to Swiss-Prot)

• Paralogous protein(s): MotP

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:
  • the interaction with DgrA inhibits MotA activity PubMed

• Interactions:
  • MotA-MotB
  • DgrA-MotA PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU13690

• Structure:

• UniProt: P28611

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: motA-motB PubMed

• Expression browser: motA PubMed

• Sigma factor: SigD PubMed
• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 100 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 613 PubMed

Biological materials

• Mutant:
  • 1A631 ( motA::erm), PubMed, available at BGSC
  • 1A923 ( motA::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: