RbgA

• Description: assembly of the 50S subunit of the ribosome
  
  

Gene name	rbgA
Synonyms	ylqF
Essential	yes PubMed
Product	GTPase
Function	ribosome assembly
Gene expression levels in SubtiExpress: rbgA
Interactions involving this protein in SubtInteract: RbgA
MW, pI	31 kDa, 9.528
Gene length, protein length	846 bp, 282 aa
Immediate neighbours	rplS, rnhB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, GTP-binding proteins

The gene

Basic information

• Locus tag: BSU16050

Phenotypes of a mutant

• essential PubMed
• depletion confers a slow growth phenotype, and induces an abnormality of the in vivo ribosome profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes PubMed
• premature 45S particles accumulated in RbgA-depleted cells lack several proteins, including L16, L27, L28, L33, L35 and L36 PubMed

Database entries

• BsubCyc: BSU16050

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • proper positioning of L6 in the large subunit of the ribosome prior to incorporation of L16 PubMed
  • Binds and hydrolyzes GTP and readily exchanges GDP for GTP

• Protein family: MTG1 subfamily (according to Swiss-Prot) Era/Obg family

• Paralogous protein(s): Era, Obg, YphC, YsxC, RbgA, YqeH, ThdF, YyaF, YnbA

Extended information on the protein

• Kinetic information:

• Domains:
  • RNA-binding domain at the C-terminus PubMed
• Modification:

• Cofactors:

• Effectors of protein activity:
  • GTPase activity requires binding of L16 to the 50S subunit of the ribosome PubMed

• Interactions:
  • RbgA-RplF PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU16050

• Structure: 1PUJ

• UniProt: O31743

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: rbgA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 94 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 568 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

References

Reviews

Natalie Verstraeten, Maarten Fauvart, Wim Versées, Jan Michiels
The universally conserved prokaryotic GTPases.
Microbiol Mol Biol Rev: 2011, 75(3);507-42, second and third pages of table of contents
[PubMed:21885683] [WorldCat.org] [DOI] (I p)

Robert A Britton
Role of GTPases in bacterial ribosome assembly.
Annu Rev Microbiol: 2009, 63;155-76
[PubMed:19575570] [WorldCat.org] [DOI] (I p)

Original publications

Megha Gulati, Nikhil Jain, Joseph H Davis, James R Williamson, Robert A Britton
Functional interaction between ribosomal protein L6 and RbgA during ribosome assembly.
PLoS Genet: 2014, 10(10);e1004694
[PubMed:25330043] [WorldCat.org] [DOI] (I e)

Ahmad Jomaa, Nikhil Jain, Joseph H Davis, James R Williamson, Robert A Britton, Joaquin Ortega
Functional domains of the 50S subunit mature late in the assembly process.
Nucleic Acids Res: 2014, 42(5);3419-35
[PubMed:24335279] [WorldCat.org] [DOI] (I p)

Ningning Li, Yuling Chen, Qiang Guo, Yixiao Zhang, Yi Yuan, Chengying Ma, Haiteng Deng, Jianlin Lei, Ning Gao
Cryo-EM structures of the late-stage assembly intermediates of the bacterial 50S ribosomal subunit.
Nucleic Acids Res: 2013, 41(14);7073-83
[PubMed:23700310] [WorldCat.org] [DOI] (I p)

Megha Gulati, Nikhil Jain, Baskaran Anand, Balaji Prakash, Robert A Britton
Mutational analysis of the ribosome assembly GTPase RbgA provides insight into ribosome interaction and ribosome-stimulated GTPase activation.
Nucleic Acids Res: 2013, 41(5);3217-27
[PubMed:23325847] [WorldCat.org] [DOI] (I p)

David Achila, Megha Gulati, Nikhil Jain, Robert A Britton
Biochemical characterization of ribosome assembly GTPase RbgA in Bacillus subtilis.
J Biol Chem: 2012, 287(11);8417-23
[PubMed:22267738] [WorldCat.org] [DOI] (I p)

Laura Schaefer, William C Uicker, Catherine Wicker-Planquart, Anne-Emmanuelle Foucher, Jean-Michel Jault, Robert A Britton
Multiple GTPases participate in the assembly of the large ribosomal subunit in Bacillus subtilis.
J Bacteriol: 2006, 188(23);8252-8
[PubMed:16997968] [WorldCat.org] [DOI] (P p)

William C Uicker, Laura Schaefer, Robert A Britton
The essential GTPase RbgA (YlqF) is required for 50S ribosome assembly in Bacillus subtilis.
Mol Microbiol: 2006, 59(2);528-40
[PubMed:16390447] [WorldCat.org] [DOI] (P p)

Takuya Morimoto, Pek Chin Loh, Tomohiro Hirai, Kei Asai, Kazuo Kobayashi, Shigeki Moriya, Naotake Ogasawara
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading): 2002, 148(Pt 11);3539-3552
[PubMed:12427945] [WorldCat.org] [DOI] (P p)