CheR

• Description: MCPs methyltransferase
  
  

Gene name	cheR
Synonyms
Essential	no
Product	MCPs methyltransferase
Function	motility, chemotaxis
Gene expression levels in SubtiExpress: cheR
MW, pI	29 kDa, 9.253
Gene length, protein length	768 bp, 256 aa
Immediate neighbours	aroF, ndk
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, motility and chemotaxis

The gene

Basic information

• Locus tag: BSU22720

Database entries

• BsubCyc: BSU22720

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: S-adenosyl-L-methionine + protein L-glutamate = S-adenosyl-L-homocysteine + protein L-glutamate methyl ester (according to Swiss-Prot)

• Protein family: Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU22720

• Structure:

• UniProt: P31105

• KEGG entry: [2]

• E.C. number: 2.1.1.80

Expression and regulation

• Operon:

• Expression browser: cheR PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • in minimal medium, CheR is present with 1,100 +/- 280 molecules per cell PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 128 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 302 PubMed

Biological materials

• Mutant:
  • 1A830 ( cheR::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Gerald L Hazelbauer, Wing-Cheung Lai
Bacterial chemoreceptors: providing enhanced features to two-component signaling.
Curr Opin Microbiol: 2010, 13(2);124-32
[PubMed:20122866] [WorldCat.org] [DOI] (I p)