yqeH

168

assembly/ stability of the 30S subunit of the ribosome, assembly of the 70S ribosome

Locus: BSU_25670

Molecular weight: 40.84 kDa

Isoelectric point: 6.01

Protein length: 366 aa

Gene length: 1101 bp

Function: ribosome assembly

Product: GTPase

Essential: no

Synonyms: yqeH

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1161 (Galperin et al., 2021)

Gene

Coordinates: 2,645,490  2,646,590

Phenotypes of a mutant

• essential PubMed, non-essential according to PubMed
• defective in 16S rRNA 3' processing by YqfG PubMed

The protein

Catalyzed reaction/ biological activity

• Binds and hydrolyzes GTP and readily exchanges GDP for GTP

Protein family

• TRAFAC class YlqF/YawG GTPase family (together with RbgA and CpgA, according to UniProt)

Domains

• CP-type G domain (aa 59-222) (according to UniProt)

Structure

• 3EC1 (PDB) (Geobacillus stearothermophilus) PubMed
• P54453 (AlphaFold)

Paralogous protein(s)

• YnbA, EngA, YyaF, Era, YsxC, RbgA, ThdF, Obg

Expression and Regulation

Operons

• Genes: pgpP-yqeH-aroD-yqeI-nadD-yqeK-yqeL-yqeM

  Description: PubMed

  Expression

  • expression is increased upon depletion of depletion of tRNA maturation factors rnpB or rnz PubMed

Biological materials

Mutant

• BKE25670 (yqeH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCTTACTCCTCCCACT,  downstream forward: _UP4_ATTTAAGAAAAGGGGAGAGG
• BKK25670 (yqeH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCTTACTCCTCCCACT,  downstream forward: _UP4_ATTTAAGAAAAGGGGAGAGG

Labs working on this gene/protein

• Naotake Ogasawara, Nara, Japan

References

Reviews

Loading

Original Publications

Loading