TagB

• Description: putative CDP-glycerol:glycerol phosphate glycerophosphotransferase
  
  

Gene name	tagB
Synonyms
Essential	yes PubMed
Product	putative CDP-glycerol:glycerol phosphate glycerophosphotransferase
Function	biosynthesis of teichoic acid
Gene expression levels in SubtiExpress: tagB
Metabolic function and regulation of this protein in SubtiPathways: TagB
MW, pI	44 kDa, 8.925
Gene length, protein length	1143 bp, 381 aa
Immediate neighbours	tagA, tagC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes, membrane proteins

This gene is a member of the following regulons

PhoP regulon, WalR regulon

The gene

Basic information

• Locus tag: BSU35760

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU35760

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: adds a single glycerol phosphate residue to the non-reducing end of the undecaprenol-phosphate-linked N-acetylmannosamine-β(1,4)-N-acetylglucosamine-1-phosphate PubMed

• Protein family: CDP-glycerol glycerophosphotransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane PubMed

Database entries

• BsubCyc: BSU35760

• Structure:

• UniProt: P27621

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: tagA-tagB PubMed

• Expression browser: tagB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed when phosphate is available (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription repression PubMed
  • WalR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alex Formstone, Rut Carballido-López, Philippe Noirot, Jeffery Errington, Dirk-Jan Scheffers
Localization and interactions of teichoic acid synthetic enzymes in Bacillus subtilis.
J Bacteriol: 2008, 190(5);1812-21
[PubMed:18156271] [WorldCat.org] [DOI] (I p)

Amit P Bhavsar, Michael A D'Elia, Tiffany D Sahakian, Eric D Brown
The Amino terminus of Bacillus subtilis TagB possesses separable localization and functional properties.
J Bacteriol: 2007, 189(19);6816-23
[PubMed:17660278] [WorldCat.org] [DOI] (P p)

Amit P Bhavsar, Ray Truant, Eric D Brown
The TagB protein in Bacillus subtilis 168 is an intracellular peripheral membrane protein that can incorporate glycerol phosphate onto a membrane-bound acceptor in vitro.
J Biol Chem: 2005, 280(44);36691-700
[PubMed:16150696] [WorldCat.org] [DOI] (P p)

Amit P Bhavsar, Laura K Erdman, Jeffrey W Schertzer, Eric D Brown
Teichoic acid is an essential polymer in Bacillus subtilis that is functionally distinct from teichuronic acid.
J Bacteriol: 2004, 186(23);7865-73
[PubMed:15547257] [WorldCat.org] [DOI] (P p)

Alistair Howell, Sarah Dubrac, Kasper Krogh Andersen, David Noone, Juliette Fert, Tarek Msadek, Kevin Devine
Genes controlled by the essential YycG/YycF two-component system of Bacillus subtilis revealed through a novel hybrid regulator approach.
Mol Microbiol: 2003, 49(6);1639-55
[PubMed:12950927] [WorldCat.org] [DOI] (P p)

W Liu, S Eder, F M Hulett
Analysis of Bacillus subtilis tagAB and tagDEF expression during phosphate starvation identifies a repressor role for PhoP-P.
J Bacteriol: 1998, 180(3);753-8
[PubMed:9457886] [WorldCat.org] [DOI] (P p)

C Mauël, A Bauduret, C Chervet, S Beggah, D Karamata
In Bacillus subtilis 168, teichoic acid of the cross-wall may be different from that of the cylinder: a hypothesis based on transcription analysis of tag genes.
Microbiology (Reading): 1995, 141 ( Pt 10);2379-89
[PubMed:7581998] [WorldCat.org] [DOI] (P p)

C Mauël, M Young, A Monsutti-Grecescu, S A Marriott, D Karamata
Analysis of Bacillus subtilis tag gene expression using transcriptional fusions.
Microbiology (Reading): 1994, 140 ( Pt 9);2279-88
[PubMed:7952180] [WorldCat.org] [DOI] (P p)