opuBD

168

choline and arsenocholine ABC transporter (membrane protein)

Locus: BSU_33700

Molecular weight: 23.78 kDa

Isoelectric point: 10.06

Protein length: 226 aa

Gene length: 681 bp

Function: compatible solute transport

Product: choline and arsenocholine ABC transporter (membrane protein)

Essential: no

Synonyms: opuBD, proZ

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Lifestyles, Coping with stress, Coping with hyper-osmotic stress

Groups of genes, Membrane proteins

List of homologs in different organisms, belongs to COG1174 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, GbsR regulon, OpcR regulon, RemA regulon

Gene

Coordinates: 3,459,806  3,460,486

The protein

Catalyzed reaction/ biological activity

uptake of choline and arsenocholine PubMed

Protein family

Binding-protein-dependent transport system permease family (according to UniProt)
CysTW subfamily (according to UniProt)

Domains

ABC transmembrane type-1 domain (aa 22-202) (according to UniProt)

Structure

P39775 (AlphaFold)

Paralogous protein(s)

OpuCD

Localization

cell membrane PubMed

Expression and Regulation

Operons

Genes: opuBA-opuBB-opuBC-opuBD

Description: PubMed

Regulation

induced by choline (GbsR) PubMed

Regulatory mechanism

GbsR: repression, (transcriptional roadblock) PubMed, in gbsR regulon
OpcR: repression, (sterical interference with RNA polymerase access) PubMed, in opcR regulon
RemA: activation, PubMed, in remA regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Additional information

An antisense RNA is predicted for'opuBD' PubMed

Biological materials

Mutant

BKE33700 (opuBD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTTCATTGACCTCTTCC,  downstream forward: _UP4_TAGAATAGAAAAAGAGGCTG
BKK33700 (opuBD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTTCATTGACCTCTTCC,  downstream forward: _UP4_TAGAATAGAAAAAGAGGCTG

References

Reviews

Loading

Original Publications

Loading