PheT

• Description: phenylalanyl-tRNA synthetase (beta subunit)
  
  

Gene name	pheT
Synonyms
Essential	yes PubMed
Product	phenylalanyl-tRNA synthetase (beta subunit)
Function	translation
Gene expression levels in SubtiExpress: pheT
Interactions involving this protein in SubtInteract: PheT
Metabolic function and regulation of this protein in SubtiPathways: pheT
MW, pI	87 kDa, 4.864
Gene length, protein length	2412 bp, 804 aa
Immediate neighbours	rnhC, pheS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, most abundant proteins

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU28630

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU28630

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe) (according to Swiss-Prot)

• Protein family: Type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions: PheS-PheT

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU28630

• Structure: 1PYS (from Thermus thermophilus hb8 mutant, 37% identity, 56% similarity) PubMed

• UniProt: P17922

• KEGG entry: [3]

• E.C. number: 6.1.1.20

Expression and regulation

• Operon: pheS-pheT PubMed

• Expression browser: pheT PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by phenylalanine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1145 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2843 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)

A A Brakhage, M Wozny, H Putzer
Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes.
Biochimie: 1990, 72(10);725-34
[PubMed:2127701] [WorldCat.org] [DOI] (P p)