Difference between revisions of "Rph"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 274 {{PubMed|24696501}}
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 1204 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:50, 17 April 2014

  • Description: RNase PH, 3'-5' exoribonuclease

Gene name rph
Synonyms
Essential no
Product RNase PH (EC 2.7.7.56)
Function 3'-5' exoribonuclease
Gene expression levels in SubtiExpress: rph
MW, pI 26 kDa, 4.888
Gene length, protein length 735 bp, 245 aa
Immediate neighbours ysnA, gerM
Sequences Protein DNA DNA_with_flanks
Genetic context
Rph context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Rph expression.png
























Categories containing this gene/protein

Rnases, translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU28370

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • major player in the exonucleolytic maturation of CCA-containing tRNA precursors PubMed
    • responsible for endonucleolytic cleavage of scRNA (scr) PubMed
  • Protein family: RNase PH family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 274 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1204 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Ciaran Condon, IBPC, Paris, France Homepage

Your additional remarks

References