YrvO

• Description: cysteine desulfurase involved in tRNA thiolation
  
  

Gene name	yrvO
Synonyms	nifS, iscS
Essential	yes PubMed
Product	cysteine desulfurase
Function	tRNA modification
Gene expression levels in SubtiExpress: yrvO
MW, pI	41 kDa, 5.463
Gene length, protein length	1137 bp, 379 aa
Immediate neighbours	trmU, cymR
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

essential genes, translation, phosphoproteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU27510

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU27510

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): NifS, NifZ, SufS, YcbU

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-385 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU27510

• Structure: 1EG5 (from Thermotoga maritima, 37% identity, 54% similarity) PubMed

• UniProt: O34599

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cymR-yrvO-trmU PubMed

• Expression browser: yrvO PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 184 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 444 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: