Difference between revisions of "Sda"

From SubtiWiki
Jump to: navigation, search
Line 47: Line 47:
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
 +
{{SubtiWiki regulon|[[DnaA regulon]]}},
 
{{SubtiWiki regulon|[[LexA regulon]]}}
 
{{SubtiWiki regulon|[[LexA regulon]]}}
  
Line 92: Line 93:
 
* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
 
** [[KinB]]-[[Sda]] {{PubMed|19465772}}
 
** [[KinB]]-[[Sda]] {{PubMed|19465772}}
** [[KinA]]-[[Sda]] {{PubMed|15023339}}
+
** [[KinA]]-[[Sda]] {{PubMed|15023339}}
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
Line 108: Line 109:
  
 
=== Additional information===
 
=== Additional information===
 
+
* [[Sda]] is rapidly degraded by the [[ClpP|ClpP/X]] system because of some uncharged residues at its C-terminus {{PubMed|16796683}}
Sda is rapidly turned over by the [[ClpP|ClpP/X]] system because of some uncharged residues at its C-terminus {{PubMed|16796683}}.
 
  
 
=Expression and regulation=
 
=Expression and regulation=
Line 123: Line 123:
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
** probably activated by [[DnaA]] under replication stress {{PubMed|17932079}}
+
** [[DnaA]]: transcription activation under replication stress {{PubMed|17932079}}
 
** [[LexA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16267290 PubMed]
 
** [[LexA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16267290 PubMed]
  
Line 155: Line 155:
 
<pubmed> 22933559</pubmed>
 
<pubmed> 22933559</pubmed>
 
== Original publications ==
 
== Original publications ==
<pubmed>11207367, 16267290, 19465772, 15023339 16796683 19684115 17932079 17350039 19101565 20525796</pubmed>
+
<pubmed>11207367, 16267290, 19465772, 15023339 16796683 19684115 17932079 17350039 19101565 20525796 26020636</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:20, 1 June 2015

  • Description: developmental checkpoint protein, inhibits the autokinase activity of KinA and KinB, control of the phosphorelay


Gene name sda
Synonyms
Essential no
Product developmental checkpoint protein
Function mediates a developmental checkpoint coupling
initiation of sporulation (phosphorylation of Spo0A)
to the function of replication initiation proteins
Gene expression levels in SubtiExpress: sda
Interactions involving this protein in SubtInteract: Sda
Function and regulation of this protein in SubtiPathways:
sda
MW, pI 6 kDa, 5.605
Gene length, protein length 156 bp, 52 aa
Immediate neighbours yloV, yqeF
Sequences Protein DNA DNA_with_flanks
Genetic context
Sda context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Sda expression.png
















Categories containing this gene/protein

phosphorelay

This gene is a member of the following regulons

DnaA regulon, LexA regulon

The gene

Basic information

  • Locus tag: BSU25690

Phenotypes of a mutant

  • sporulates at a higher frequency
  • will sporulate in the presence of replication stress

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Sda is a checkpoint protein that is upregulated in response to replication stress PubMed. Sda inhibits the autokinase activity of KinA (and likely KinB as well). This in its turn results in reduced levels of Spo0A~P. Thus, replication stressed cells will not initiate sporulation.
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

  • Sda is rapidly degraded by the ClpP/X system because of some uncharged residues at its C-terminus PubMed

Expression and regulation

  • Regulatory mechanism:
    • DnaA: transcription activation under replication stress PubMed
    • LexA: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant:
    • 1A1034 (no resistance), PubMed, available at BGSC
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Bill Burkholder Homepage

Your additional remarks

References

Reviews

Original publications