trpB

168

tryptophan synthase (beta subunit)

Locus: BSU_22640

Molecular weight: 43.42 kDa

Isoelectric point: 5.53

Protein length: 400 aa

Gene length: 1200 bp

Function: biosynthesis of tryptophan

Product: tryptophan synthase (beta subunit)

Essential: no

E.C.: 4.2.1.20

Synonyms: trpB

Genomic Context

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List of homologs in different organisms, belongs to COG0133 (Galperin et al., 2021)

Gene

Coordinates: 2,372,304 → 2,373,506

The protein

Catalyzed reaction/ biological activity

• (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine --> D-glyceraldehyde 3-phosphate + H₂O + L-tryptophan (according to UniProt)

Protein family

• TrpB family (single member, according to UniProt)

Cofactors

• PLP (according to UniProt)

Structure

• 4NEG (PDB) (from Bacillus anthracis, 60% identity)
• P07600 (AlphaFold)

Expression and Regulation

Operons

• Genes: trpE-trpD-trpC-trpF-trpB-trpA

  Description: PubMed

  Regulation

  • not expressed if tryptophan is available (MtrB) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
  • the RNA switch RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • MtrB: transcription termination, PubMed, in mtrB regulon

• Genes: trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE

  Description: PubMed

  Regulation

  • not expressed if tryptophan is available (MtrB) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

  Regulatory mechanism

  • MtrB: transcription termination, PubMed, in mtrB regulon

Biological materials

Mutant

• BKE22640 (ΔtrpB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCCAAAATCACCGTATCTGC,  downstream forward: _UP4_GTATTGGAAGAAGAGGTGAA
• BKK22640 (ΔtrpB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCCAAAATCACCGTATCTGC,  downstream forward: _UP4_GTATTGGAAGAAGAGGTGAA

References

Reviews

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Original Publications

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