CpgA

• Description: GTPase, activity stimulated by ribosomes, may be involved in ribosome maturation
  
  

Gene name	cpgA
Synonyms	yloQ
Essential	no
Product	GTPase
Function	ribosome assembly, coordination of peptidoglycan deposition in the cell wall
Gene expression levels in SubtiExpress: cpgA
Metabolic function and regulation of this protein in SubtiPathways: cpgA
MW, pI	33 kDa, 4.743
Gene length, protein length	894 bp, 298 aa
Immediate neighbours	prkC, rpe
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall/ other, translation, GTP-binding proteins, phosphoproteins

The gene

Basic information

• Locus tag: BSU15780

Phenotypes of a mutant

• forms curly cells PubMed

Database entries

• BsubCyc: BSU15780

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: engC GTPase domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • in vitro phosphorylated on Thr-166 by PrkC, dephosphorylated by PrpC PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • binds the 30S subunit of the ribosome PubMed

• Localization:

Database entries

• BsubCyc: BSU15780

• Structure: 1T9H

• UniProt: O34530

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
  • prpC-prkC-cpgA PubMed
  • cpgA PubMed

• Expression browser: cpgA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 170 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 265 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Tony Wilkinson, York University, U.K. homepage

References

Reviews

Robert A Britton
Role of GTPases in bacterial ribosome assembly.
Annu Rev Microbiol: 2009, 63;155-76
[PubMed:19575570] [WorldCat.org] [DOI] (I p)