Difference between revisions of "MurF"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 547 {{PubMed|24696501}}
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 914 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:31, 17 April 2014

  • Description: UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine synthetase

Gene name murF
Synonyms ydbQ
Essential yes PubMed
Product UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-
2,6- diaminopimeloyl-D-alanyl-D-alanine synthetase
Function peptidoglycan precursor biosynthesis
Gene expression levels in SubtiExpress: murF
Metabolic function and regulation of this protein in SubtiPathways:
murF
MW, pI 49 kDa, 5.337
Gene length, protein length 1371 bp, 457 aa
Immediate neighbours ddl, cshA
Sequences Protein DNA DNA_with_flanks
Genetic context
MurF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MurF expression.png































Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

  • Locus tag: BSU04570

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine (according to Swiss-Prot)
  • Protein family: murCDEF family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1GG4 (from Escherichia coli, 32% identity, 50% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 547 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 914 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

Jean van Heijenoort
Lipid intermediates in the biosynthesis of bacterial peptidoglycan.
Microbiol Mol Biol Rev: 2007, 71(4);620-35
[PubMed:18063720] [WorldCat.org] [DOI] (P p)