SasB

• Description: (p)ppGpp synthetase
  
  

Gene name	sasB
Synonyms	yjbM
Essential	no
Product	(p)ppGpp synthetase
Function	ppGpp synthesis independent from stringent response
Gene expression levels in SubtiExpress: sasB
MW, pI	24 kDa, 6.708
Gene length, protein length	633 bp, 211 aa
Immediate neighbours	yjbL, nadF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides

The gene

Basic information

• Locus tag: BSU11600

Phenotypes of a mutant

• a relA sasA sasB triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of guaB or inactivation of codY PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): SasA

Extended information on the protein

• Kinetic information:

• Domains: single ppGpp synthetase domain PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31611

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yjbL-sasB-nadF-yjbO PubMed

• Expression browser: sasB PubMed

• Sigma factor:

• Regulation: expressed during exponential growth

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 169 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Mariangela Tabone, Virginia S Lioy, Silvia Ayora, Cristina Machón, Juan C Alonso
Role of toxin ζ and starvation responses in the sensitivity to antimicrobials.
PLoS One: 2014, 9(1);e86615
[PubMed:24489751] [WorldCat.org] [DOI] (I e)

Allison Kriel, Shaun R Brinsmade, Jessica L Tse, Ashley K Tehranchi, Alycia N Bittner, Abraham L Sonenshein, Jue D Wang
GTP dysregulation in Bacillus subtilis cells lacking (p)ppGpp results in phenotypic amino acid auxotrophy and failure to adapt to nutrient downshift and regulate biosynthesis genes.
J Bacteriol: 2014, 196(1);189-201
[PubMed:24163341] [WorldCat.org] [DOI] (I p)

Kazumi Tagami, Hideaki Nanamiya, Yuka Kazo, Marie Maehashi, Shota Suzuki, Eri Namba, Masahiro Hoshiya, Ryo Hanai, Yuzuru Tozawa, Takuya Morimoto, Naotake Ogasawara, Yasushi Kageyama, Katsutoshi Ara, Katsuya Ozaki, Masaki Yoshida, Haruko Kuroiwa, Tsuneyoshi Kuroiwa, Yoshiaki Ohashi, Fujio Kawamura
Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp(0) mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome.
Microbiologyopen: 2012, 1(2);115-34
[PubMed:22950019] [WorldCat.org] [DOI] (I p)

Yousuke Natori, Kazumi Tagami, Kana Murakami, Sawako Yoshida, Osamu Tanigawa, Yoonsuh Moh, Kenta Masuda, Tetsuya Wada, Shota Suzuki, Hideaki Nanamiya, Yuzuru Tozawa, Fujio Kawamura
Transcription activity of individual rrn operons in Bacillus subtilis mutants deficient in (p)ppGpp synthetase genes, relA, yjbM, and ywaC.
J Bacteriol: 2009, 191(14);4555-61
[PubMed:19447912] [WorldCat.org] [DOI] (I p)

Anjana Srivatsan, Yi Han, Jianlan Peng, Ashley K Tehranchi, Richard Gibbs, Jue D Wang, Rui Chen
High-precision, whole-genome sequencing of laboratory strains facilitates genetic studies.
PLoS Genet: 2008, 4(8);e1000139
[PubMed:18670626] [WorldCat.org] [DOI] (I e)

Hideaki Nanamiya, Koji Kasai, Akira Nozawa, Choong-Soo Yun, Takakuni Narisawa, Kana Murakami, Yousuke Natori, Fujio Kawamura, Yuzuru Tozawa
Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis.
Mol Microbiol: 2008, 67(2);291-304
[PubMed:18067544] [WorldCat.org] [DOI] (P p)