SpoIIIJ

• Description: Sec-independent membrane protein translocase, essential for SigG activity at stage III
  
  

Gene name	spoIIIJ
Synonyms	spo0J87
Essential	no
Product	membrane protein translocase
Function	membrane insertion of proteins and protein secretion
Gene expression levels in SubtiExpress: spoIIIJ
Interactions involving this protein in SubtInteract: SpoIIIJ
MW, pI	29 kDa, 9.992
Gene length, protein length	783 bp, 261 aa
Immediate neighbours	jag, rnpA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein secretion, sporulation/ other, membrane proteins

The gene

Basic information

• Locus tag: BSU41040

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: membrane protein translocase, facilitates insertion of SpoIIIAE into the membrane PubMed

• Protein family: Type 2 subfamily (according to Swiss-Prot)

• Paralogous protein(s): YidC2, one of the two proteins has to be present for viability PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • SpoIIIAE-SpoIIIJ PubMed
  • SpoIIIJ-MifM PubMed

• Localization:
  • membrane PubMed

Database entries

• Structure:

• UniProt: Q01625

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIIIJ-jag PubMed

• Expression browser: spoIIIJ PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Richard Losick, Harvard University, Cambridge, USA homepage

Adriano Henriques, Lisbon, Portugal homepage

References

Reviews

Ross E Dalbey, Peng Wang, Jan Maarten van Dijl
Membrane proteases in the bacterial protein secretion and quality control pathway.
Microbiol Mol Biol Rev: 2012, 76(2);311-30
[PubMed:22688815] [WorldCat.org] [DOI] (I p)