Difference between revisions of "Fbp"

From SubtiWiki
Jump to: navigation, search
Line 37: Line 37:
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
<br/><br/><br/><br/>
+
<br/><br/>
<br/><br/><br/><br/>
 
 
 
 
 
<br/><br/><br/><br/><br/><br/>
 
  
 
= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
Line 64: Line 60:
  
 
=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
Line 81: Line 74:
  
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
 +
** specific activity: 0.203 µmol min<sup>-1</sup> (mg protein)<sup>-1</sup> {{PubMed|24571712}}
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
Line 112: Line 106:
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fbp_4128029_4130044_1 fbp] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fbp_4128029_4130044_1 fbp] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigA]] {{PubMed|9696785}}
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9696785}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
Line 140: Line 134:
 
=References=
 
=References=
  
<pubmed>6257649,221467,9696785,,, </pubmed>
+
<pubmed>6257649,221467,9696785,24571712,, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:37, 28 February 2014

  • Description: fructose-1,6-bisphosphatase, involved in gluconeogenesis

Gene name fbp
Synonyms fdp, yydE
Essential no
Product fructose-1,6-bisphosphatase
Function gluconeogenesis
Gene expression levels in SubtiExpress: fbp
Metabolic function and regulation of this protein in SubtiPathways:
fbp
MW, pI 77 kDa, 5.601
Gene length, protein length 2013 bp, 671 aa
Immediate neighbours yydF, yydD
Sequences Protein DNA DNA_with_flanks
Genetic context
Fbp context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Fbp expression.png















Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU40190

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate (according to Swiss-Prot)
  • Protein family: FBPase class 3 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
    • specific activity: 0.203 µmol min-1 (mg protein)-1 PubMed
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Michael Kohlstedt, Praveen K Sappa, Hanna Meyer, Sandra Maaß, Adrienne Zaprasis, Tamara Hoffmann, Judith Becker, Leif Steil, Michael Hecker, Jan Maarten van Dijl, Michael Lalk, Ulrike Mäder, Jörg Stülke, Erhard Bremer, Uwe Völker, Christoph Wittmann
Adaptation of Bacillus subtilis carbon core metabolism to simultaneous nutrient limitation and osmotic challenge: a multi-omics perspective.
Environ Microbiol: 2014, 16(6);1898-917
[PubMed:24571712] [WorldCat.org] [DOI] (I p)

Y Fujita, K Yoshida, Y Miwa, N Yanai, E Nagakawa, Y Kasahara
Identification and expression of the Bacillus subtilis fructose-1, 6-bisphosphatase gene (fbp).
J Bacteriol: 1998, 180(16);4309-13
[PubMed:9696785] [WorldCat.org] [DOI] (P p)

Y Fujita, E Freese
Isolation and properties of a Bacillus subtilis mutant unable to produce fructose-bisphosphatase.
J Bacteriol: 1981, 145(2);760-7
[PubMed:6257649] [WorldCat.org] [DOI] (P p)

Y Fujita, E Freese
Purification and properties of fructose-1,6-bisphosphatase of Bacillus subtilis.
J Biol Chem: 1979, 254(12);5340-9
[PubMed:221467] [WorldCat.org] (P p)