Difference between revisions of "NifZ"
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29590&redirect=T BSU29590] | ||
* '''DBTBS entry:''' no entry | * '''DBTBS entry:''' no entry | ||
| Line 92: | Line 93: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29590&redirect=T BSU29590] | ||
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1EG5 1EG5] (NifS-like protein from Thermotoga maritima, 35% identity, 55% similarity) {{PubMed|10715213}} | * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1EG5 1EG5] (NifS-like protein from Thermotoga maritima, 35% identity, 55% similarity) {{PubMed|10715213}} | ||
Revision as of 14:31, 2 April 2014
- Description: cysteine desulfurase, biosynthesis of 4-thiouridine in tRNA
| Gene name | nifZ |
| Synonyms | iscSB |
| Essential | no |
| Product | cysteine desulfurase |
| Function | biosynthesis of 4-thiouridine in tRNA |
| Gene expression levels in SubtiExpress: nifZ | |
| Interactions involving this protein in SubtInteract: NifZ | |
| Metabolic function and regulation of this protein in SubtiPathways: nifZ | |
| MW, pI | 41 kDa, 6.34 |
| Gene length, protein length | 1143 bp, 381 aa |
| Immediate neighbours | thiI, braB |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29590
Phenotypes of a mutant
Database entries
- BsubCyc: BSU29590
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: removes sulfuryl group from cysteine and transfers it to ThiI PubMed
- Protein family:
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU29590
- UniProt: O34874
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578]
[WorldCat.org]
[DOI]
(I p)
Original publications
Lauren J Rajakovich, John Tomlinson, Patricia C Dos Santos
Functional Analysis of Bacillus subtilis Genes Involved in the Biosynthesis of 4-Thiouridine in tRNA.
J Bacteriol: 2012, 194(18);4933-40
[PubMed:22773787]
[WorldCat.org]
[DOI]
(I p)
J T Kaiser, T Clausen, G P Bourenkow, H D Bartunik, S Steinbacher, R Huber
Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assembly.
J Mol Biol: 2000, 297(2);451-64
[PubMed:10715213]
[WorldCat.org]
[DOI]
(P p)
