Difference between revisions of "YtsJ"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[utilization of specific carbon sources]]}} | + | {{SubtiWiki category|[[utilization of specific carbon sources]]}}, |
| + | [[most abundant proteins]] | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| Line 62: | Line 63: | ||
=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| − | |||
=The protein= | =The protein= | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' | + | * '''[[SubtInteract|Interactions]]:''' |
| − | * '''Localization:''' Cytoplasm (Homogeneous) | + | * '''[[Localization]]:''' |
| + | ** Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] | ||
=== Database entries === | === Database entries === | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ytsJ_2989900_2991132_-1 ytsJ] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ytsJ_2989900_2991132_-1 ytsJ] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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* '''Additional information:''' | * '''Additional information:''' | ||
| + | ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}} | ||
=Biological materials = | =Biological materials = | ||
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=References= | =References= | ||
| − | + | <pubmed>16479537, 16788182 ,12949160 9387221 23136871 22740702 15378759</pubmed> | |
| − | <pubmed>16479537, 16788182 ,12949160 9387221 23136871</pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:01, 5 March 2014
- Description: malic enzyme, forms a transhydrogenation cycle with MalS for balancing of NADPH
| Gene name | ytsJ |
| Synonyms | |
| Essential | no |
| Product | NADP-dependent malate dehydrogenase |
| Function | malate utilization |
| Gene expression levels in SubtiExpress: ytsJ | |
| Metabolic function and regulation of this protein in SubtiPathways: ytsJ | |
| MW, pI | 43 kDa, 5.046 |
| Gene length, protein length | 1230 bp, 410 aa |
| Immediate neighbours | accD, dnaE |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
utilization of specific carbon sources, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29220
Phenotypes of a mutant
Poor growth with malate as single carbon source PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-malate + NADP+ = pyruvate + CO2 + NADPH (according to Swiss-Prot) malate <--> pyruvate
- Protein family: malic enzymes family (according to Swiss-Prot)
- Paralogous protein(s): MleA
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization:
- Cytoplasm (Homogeneous) PubMed
Database entries
- Structure: 1WW8 (from Pyrococcus horikoshii, 46% identity, 63% similarity)
- UniProt: O34962
- KEGG entry: [3]
- E.C. number: 1.1.1.38
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- belongs to the 100 most abundant proteins PubMed
Biological materials
- Mutant:
- GP612 (spc), available in Jörg Stülke's lab
- GP1143 (spc), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- two-hybrid system:
- FLAG-tag construct: GP1131 (spc, based on pGP1331), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Your additional remarks
References
Frederik M Meyer, Jörg Stülke
Malate metabolism in Bacillus subtilis: distinct roles for three classes of malate-oxidizing enzymes.
FEMS Microbiol Lett: 2013, 339(1);17-22
[PubMed:23136871]
[WorldCat.org]
[DOI]
(I p)
Martin Rühl, Dominique Le Coq, Stéphane Aymerich, Uwe Sauer
13C-flux analysis reveals NADPH-balancing transhydrogenation cycles in stationary phase of nitrogen-starving Bacillus subtilis.
J Biol Chem: 2012, 287(33);27959-70
[PubMed:22740702]
[WorldCat.org]
[DOI]
(I p)
Guillaume Lerondel, Thierry Doan, Nicola Zamboni, Uwe Sauer, Stéphane Aymerich
YtsJ has the major physiological role of the four paralogous malic enzyme isoforms in Bacillus subtilis.
J Bacteriol: 2006, 188(13);4727-36
[PubMed:16788182]
[WorldCat.org]
[DOI]
(P p)
Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537]
[WorldCat.org]
[DOI]
(P p)
Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759]
[WorldCat.org]
[DOI]
(P p)
Thierry Doan, Pascale Servant, Shigeo Tojo, Hirotake Yamaguchi, Guillaume Lerondel, Ken-Ichi Yoshida, Yasutaro Fujita, Stéphane Aymerich
The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate.
Microbiology (Reading): 2003, 149(Pt 9);2331-2343
[PubMed:12949160]
[WorldCat.org]
[DOI]
(P p)
Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221]
[WorldCat.org]
[DOI]
(P p)
