Difference between revisions of "SpeD"

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* '''Description:''' S-adenosylmethionine decarboxylase <br/><br/>
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* '''Description:''' S-adenosylmethionine decarboxylase, required for [[biofilm formation]]  <br/><br/>
  
 
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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[miscellaneous metabolic pathways]]}}
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{{SubtiWiki category|[[miscellaneous metabolic pathways]]}},
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{{SubtiWiki category|[[biofilm formation]]}}
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* no [[biofilm formation]] {{PubMed|24529384}}
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 18:16, 28 February 2014

Gene name speD
Synonyms ytcF
Essential no
Product S-adenosylmethionine decarboxylase
Function spermidine, polyamine biosynthesis
Gene expression levels in SubtiExpress: speD
Metabolic function and regulation of this protein in SubtiPathways:
speD
MW, pI 13 kDa, 4.768
Gene length, protein length 384 bp, 128 aa
Immediate neighbours ytcG, gapB
Sequences Protein DNA DNA_with_flanks
Genetic context
SpeD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpeD expression.png















Categories containing this gene/protein

miscellaneous metabolic pathways, biofilm formation

This gene is a member of the following regulons

CcpN regulon

The gene

Basic information

  • Locus tag: BSU29010

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: S-adenosyl-L-methionine = (5-deoxy-5-adenosyl)(3-aminopropyl)-methylsulfonium salt + CO2 (according to Swiss-Prot)
  • Protein family: Type 1 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1VR7 (from Thermotoga maritima, 47% identity, 72% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:
    • speD: the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the monocistronic speD mRNA increases from 1.4 to 36 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References