Difference between revisions of "PheS"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
 
{{SubtiWiki category|[[translation]]}},
 
{{SubtiWiki category|[[translation]]}},
 
{{SubtiWiki category|[[essential genes]]}},
 
{{SubtiWiki category|[[essential genes]]}},
{{SubtiWiki category|[[universally conserved proteins]]}}
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{{SubtiWiki category|[[universally conserved proteins]]}},
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[[most abundant proteins]]
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
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* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pheS_2929438_2930472_-1 pheS] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pheS_2929438_2930472_-1 pheS] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigA]] {{PubMed|2127701}}
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* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|2127701}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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* '''Additional information:'''
 
* '''Additional information:'''
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** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
  
<pubmed>19258532,2127701 , </pubmed>
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<pubmed>19258532,2127701 , 15378759</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:23, 5 March 2014

Gene name pheS
Synonyms
Essential yes PubMed
Product phenylalanyl-tRNA synthetase (alpha subunit)
Function translation
Gene expression levels in SubtiExpress: pheS
Interactions involving this protein in SubtInteract: PheS
Metabolic function and regulation of this protein in SubtiPathways:
pheS
MW, pI 38 kDa, 5.083
Gene length, protein length 1032 bp, 344 aa
Immediate neighbours pheT, ysgA
Sequences Protein DNA DNA_with_flanks
Genetic context
PheS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PheS expression.png















Categories containing this gene/protein

translation, essential genes, universally conserved proteins, most abundant proteins

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU28640

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe) (according to Swiss-Prot)
  • Protein family: Phe-tRNA synthetase alpha chain type 1 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 1PYS (from Thermus thermophilus hb8 mutant, 37% identity, 56% similarity) PubMed
  • KEGG entry: [3]

Additional information

universally conserved protein

Expression and regulation

  • Regulation:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)

A A Brakhage, M Wozny, H Putzer
Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes.
Biochimie: 1990, 72(10);725-34
[PubMed:2127701] [WorldCat.org] [DOI] (P p)