Difference between revisions of "GroEL"

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{{SubtiWiki category|[[heat shock proteins]]}},
 
{{SubtiWiki category|[[heat shock proteins]]}},
 
{{SubtiWiki category|[[essential genes]]}},
 
{{SubtiWiki category|[[essential genes]]}},
{{SubtiWiki category|[[phosphoproteins]]}}
+
{{SubtiWiki category|[[phosphoproteins]]}},
 +
[[most abundant proteins]]
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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* '''Additional information:'''
 
* '''Additional information:'''
 +
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
<pubmed>9303302,1350777,12082092,,17726680 16493705 9303302,1350776 ,8576042 7592421 22517742 22268681 24263382</pubmed>
+
<pubmed>9303302,1350777,12082092,15378759,17726680 16493705 9303302,1350776 ,8576042 7592421 22517742 22268681 24263382</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 13:00, 5 March 2014

  • Description: chaperonin and co-repressor for HrcA

Gene name groEL
Synonyms
Essential yes PubMed
Product chaperonin, co-repressor for HrcA
Function protein folding and re-folding
Gene expression levels in SubtiExpress: groEL
Interactions involving this protein in SubtInteract: GroEL
Metabolic function and regulation of this protein in SubtiPathways:
groEL
MW, pI 57 kDa, 4.531
Gene length, protein length 1632 bp, 544 aa
Immediate neighbours groES, ydzT/1
Sequences Protein DNA DNA_with_flanks
Genetic context
GroEL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GroEL expression.png















Categories containing this gene/protein

chaperones/ protein folding, heat shock proteins, essential genes, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

HrcA regulon

The gene

Basic information

  • Locus tag: BSU06030

Expression

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: acts as co-repressor for HrcA
  • Protein family: chaperonin (HSP60) family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
    • phosphorylated on Arg-35, Arg-116, and Arg-282 PubMed
    • phosphorylated on ser/ thr/ tyr PubMed, PubMed
  • Effectors of protein activity:
  • Localization:
    • cytoplasm (according to Swiss-Prot)
    • recruited to the membrane after ethanol stress PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism: HrcA: transcription repression PubMed

Biological materials

  • Mutant:
  • Expression vector:
    • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1000 available in Jörg Stülke's lab
    • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP1002 available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage

Your additional remarks

References