LigD

• Description: DNA repair polymerase/ ligase in non-homologous end joining DNA repair
  
  

Gene name	ligD
Synonyms	ykoU
Essential	no
Product	DNA repair polymerase/ ligase
Function	non-homologous end joining DNA repair, repair of gapped DNA substrates
Gene expression levels in SubtiExpress: ligD
Interactions involving this protein in SubtInteract: LigD
MW, pI	70 kDa, 6.646
Gene length, protein length	1833 bp, 611 aa
Immediate neighbours	ykoT, ykoV
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

The gene

Basic information

• Locus tag: BSU13400

Phenotypes of a mutant

• sensitivity to ionizing radiation in the stationary phase PubMed
• sensitivity of spores to several DNA-damaging treatments known to cause double strand breaks, such as UV-ray, X-ray, ultrahigh vacuum and wet heat PubMed
• a ykoV-ligD double mutant is sensitive to radiation PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • N-terminal DNA ligase catalytic domain (aa 1 - 331) linked to a C-terminal polymerase domain (aa 332 - 611) PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • YkoV-LigD PubMed

• Localization:

Database entries

• Structure:

• UniProt: O34398

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ykoV-ligD PubMed

• Expression browser: ligD PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed during sporulation in the forespore (SigG, SpoVT) PubMed

• Regulatory mechanism:
  • SpoVT: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • BP141 (ykoV-ligD::kan) available in Fabian Commichau's lab
  • BP142 (ykoW-ykoV-ligD::kan) available in Fabian Commichau's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Stewart Shuman, Michael S Glickman
Bacterial DNA repair by non-homologous end joining.
Nat Rev Microbiol: 2007, 5(11);852-61
[PubMed:17938628] [WorldCat.org] [DOI] (I p)