FtsE

• Description: ABC transporter (ATP-binding protein) (FtsE-FtsX), required for CwlO activity (cell elongation) and for proper activation of Spo0A and initiation of sporulation
  
  

Gene name	ftsE
Synonyms
Essential	no
Product	ABC transporter (ATP-binding protein)
Function	control of cell wall synthesis
Gene expression levels in SubtiExpress: ftsE
Interactions involving this protein in SubtInteract: FtsE
MW, pI	25 kDa, 8.419
Gene length, protein length	684 bp, 228 aa
Immediate neighbours	ftsX, cccB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, cell wall synthesis, phosphorelay, membrane proteins

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU35260

Phenotypes of a mutant

• a ftsE mutation is synthetically lethal with a lytE mutation (due to a lack of autolysin activity) PubMed
• shorter, fatter cells, this can be rescued by addition of Mg(2+) PubMed
• reduced growth rate, especially at low Mg(2+) concentrations PubMed

Database entries

• BsubCyc: BSU35260

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • necessary for the autolysin activity of CwlO PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • FtsE-FtsX PubMed

• Localization: membrane associated (via FtsX) PubMed

Database entries

• BsubCyc: BSU35260

• Structure: 3TIF (ATP-binding protein from Methanococcus jannaschii, 40% identity) PubMed

• UniProt: O34814

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ftsE-ftsX (according to DBTBS)

• Expression browser: ftsE PubMed

• Sigma factor:

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Patricia Domínguez-Cuevas, Ida Porcelli, Richard A Daniel, Jeff Errington
Differentiated roles for MreB-actin isologues and autolytic enzymes in Bacillus subtilis morphogenesis.
Mol Microbiol: 2013, 89(6);1084-98
[PubMed:23869552] [WorldCat.org] [DOI] (I p)

Jeffrey Meisner, Paula Montero Llopis, Lok-To Sham, Ethan Garner, Thomas G Bernhardt, David Z Rudner
FtsEX is required for CwlO peptidoglycan hydrolase activity during cell wall elongation in Bacillus subtilis.
Mol Microbiol: 2013, 89(6);1069-83
[PubMed:23855774] [WorldCat.org] [DOI] (I p)

Maria E Zoghbi, Kerry L Fuson, Roger B Sutton, Guillermo A Altenberg
Kinetics of the association/dissociation cycle of an ATP-binding cassette nucleotide-binding domain.
J Biol Chem: 2012, 287(6);4157-64
[PubMed:22158619] [WorldCat.org] [DOI] (I p)

Sharon Garti-Levi, Ronen Hazan, James Kain, Masaya Fujita, Sigal Ben-Yehuda
The FtsEX ABC transporter directs cellular differentiation in Bacillus subtilis.
Mol Microbiol: 2008, 69(4);1018-28
[PubMed:18573177] [WorldCat.org] [DOI] (I p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)