Difference between revisions of "SpoIIIE"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16800&redirect=T BSU16800]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spoIIIE.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spoIIIE.html]
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16800&redirect=T BSU16800]
  
 
* '''Structure:'''
 
* '''Structure:'''

Revision as of 13:46, 2 April 2014

  • Description: ATP-dependent DNA translocase, resolution of chromosomal dimers after DNA replication, transports the forespore chromosome across the sporulation septum

Gene name spoIIIE
Synonyms
Essential no
Product ATP-dependent DNA translocase required for chromosome

partitioning through the septum into the forespore

Function chromosome partition during sporulation
Gene expression levels in SubtiExpress: spoIIIE
MW, pI 86 kDa, 6.757
Gene length, protein length 2361 bp, 787 aa
Immediate neighbours ylzJ, ymfC
Sequences Protein DNA DNA_with_flanks
Genetic context
SpoIIIE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpoIIIE expression.png















Categories containing this gene/protein

DNA condensation/ segregation, sporulation/ other, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU16800

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • transports the forespore chromosome across the sporulation septum PubMed
    • translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes PubMed
    • the two DNA translocases SftA and SpoIIIE synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively PubMed
    • binds DNA sequences named SRS for SpoIIIE recognition sequence, GAGAAGGG PubMed
  • Protein family:
  • Paralogous protein(s): SftA

Extended information on the protein

  • Kinetic information:
  • Domains:
    • N-terminal transmembrane domain (4 helices) PubMed
    • 134 aa linker PubMed
    • 512 aa motor domain at the C-terminus PubMed
  • Modification:
  • Effectors of protein activity:
  • Localization:
    • transmembrane domain mediates dynamic localization to active division sites PubMed
    • complexes are recruited to nascent sites of septation, and are subsequently escorted by the constriction machinery to the center of sporulation and division septa PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Xindan Wang, Paula Montero Llopis, David Z Rudner
Organization and segregation of bacterial chromosomes.
Nat Rev Genet: 2013, 14(3);191-203
[PubMed:23400100] [WorldCat.org] [DOI] (I p)

Rodrigo Reyes-Lamothe, Emilien Nicolas, David J Sherratt
Chromosome replication and segregation in bacteria.
Annu Rev Genet: 2012, 46;121-43
[PubMed:22934648] [WorldCat.org] [DOI] (I p)

Christine Kaimer, Peter L Graumann
Players between the worlds: multifunctional DNA translocases.
Curr Opin Microbiol: 2011, 14(6);719-25
[PubMed:22047950] [WorldCat.org] [DOI] (I p)

Original publications