Difference between revisions of "GabT"

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(Expression and regulation)
(Labs working on this gene/protein)
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
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[[Linc Sonenshein|Linc Sonenshein]], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
  
 
=Your additional remarks=
 
=Your additional remarks=

Revision as of 07:49, 22 November 2013

  • Description: gamma-aminobutyrate transaminase, general stress protein

Gene name gabT
Synonyms ycnG
Essential no
Product gamma-aminobutyrate transaminase
Function utilization of gamma-amino butyric acid and synthesis of norspermidine
Gene expression levels in SubtiExpress: gabT
MW, pI 47 kDa, 5.304
Gene length, protein length 1308 bp, 436 aa
Immediate neighbours gabR, gabD
Sequences Protein DNA DNA_with_flanks
Genetic context
GabT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GabT expression.png
























Categories containing this gene/protein

utilization of amino acids, miscellaneous metabolic pathways, biofilm formation

This gene is a member of the following regulons

GabR regulon

The gene

Basic information

  • Locus tag: BSU03900

Phenotypes of a mutant

    • block in norspermidine production PubMed
    • impaired biofilm disassembly PubMed
    • growth defect in the presence of gamma-aminobutyrate PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate (according to Swiss-Prot)
  • Protein family: class-III pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the presence of gamma-butyric acid and pyridoxal 5'-phosphate (GabR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Your additional remarks

References

Ilana Kolodkin-Gal, Shugeng Cao, Liraz Chai, Thomas Böttcher, Roberto Kolter, Jon Clardy, Richard Losick
A self-produced trigger for biofilm disassembly that targets exopolysaccharide.
Cell: 2012, 149(3);684-92
[PubMed:22541437] [WorldCat.org] [DOI] (I p)

Boris R Belitsky
Bacillus subtilis GabR, a protein with DNA-binding and aminotransferase domains, is a PLP-dependent transcriptional regulator.
J Mol Biol: 2004, 340(4);655-64
[PubMed:15223311] [WorldCat.org] [DOI] (P p)

Boris R Belitsky, Abraham L Sonenshein
GabR, a member of a novel protein family, regulates the utilization of gamma-aminobutyrate in Bacillus subtilis.
Mol Microbiol: 2002, 45(2);569-83
[PubMed:12123465] [WorldCat.org] [DOI] (P p)