Difference between revisions of "Methods"
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* introduction of markerfree deletions: {{PubMed|24140578,23916947,23911571,22422839,20057169}} | * introduction of markerfree deletions: {{PubMed|24140578,23916947,23911571,22422839,20057169}} | ||
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==Key references== | ==Key references== | ||
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Revision as of 18:05, 28 November 2013
Here you can find a list of methods for working with Bacillus subtilis:
Contents
- 1 Media
- 2 Analysis of protein-protein interactions
- 3 Analysis of membrane proteins
- 4 Protein expression
- 5 Strain construction
- 6 RNA analysis
- 7 Genetic work with non-standard strains
- 8 General procedures
- 9 Microscopic techniques
- 10 Transposon mutagenesis
- 11 Protein-DNA interactions
- 12 Genome engineering
- 13 Key references
Media
Analysis of protein-protein interactions
- Bacterial Two Hybrid: BACTH
- SPINE: A method to detect in vivo protein-protein interactions
- in vivo detection of protein-protein interactions using DivIVA and GFP PubMed
Analysis of membrane proteins
- application of a split green fluorescent protein reporter PubMed
Protein expression
- the LIKE system PubMed
Strain construction
- introduction of markerfree deletions: PubMed
- generation of markerfree mutations: PubMed
RNA analysis
- Mechanical cell disruption and extraction of RNA from B. subtilis: Media:SOP-RNA.pdf
Genetic work with non-standard strains
- transformation of wild-type B. subtilis strains incl. NCIB3610: PubMed
- efficient electroporation of B. subtilis PubMed
- a novel transformation protocol for B. subtilis DB104 PubMed
General procedures
- Standard operation procedures (SOPs) of the SYSMO-BACELL consortium
Microscopic techniques
- Live cell imaging of B. subtilis cells using automated time-lapse microscopy PubMed
- Assay of gene expression dynamics using live cell imaging PubMed
- Visualization and quantification of gene expression heterogeneity in growing microbial cells PubMed
- GFP variants specifically optimizd for use in B. subtilis PubMed
Transposon mutagenesis
- mariner transposon mutagenesis for random inducible-expression insertions and transcriptional reporter fusion insertions PubMed
Protein-DNA interactions
- ChAP-chip: A modified ChIP-chip protocol for the in vivo identification of binding sites of DNA-binding proteins PubMed
Genome engineering
- generalized bacterial genome editing using mobile group II introns and Cre-lox PubMed
- A review on genome engineering:
Kevin M Esvelt, Harris H Wang
Genome-scale engineering for systems and synthetic biology.
Mol Syst Biol: 2013, 9;641
[PubMed:23340847]
[WorldCat.org]
[DOI]
(I p)
Key references
