RapG

• Description: response regulator aspartate phosphatase, controls DegU activity
  
  

Gene name	rapG
Synonyms	yycM
Essential	no
Product	response regulator aspartate phosphatase
Function	control of DegU activity
Gene expression levels in SubtiExpress: rapG
Interactions involving this protein in SubtInteract: RapG
MW, pI	42 kDa, 4.807
Gene length, protein length	1095 bp, 365 aa
Immediate neighbours	yycN, phrG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, quorum sensing

This gene is a member of the following regulons

RghR regulon, SinR regulon

The gene

Basic information

• Locus tag: BSU40300

Database entries

• BsubCyc: BSU40300

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: control of DegU activity PubMed

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: binding of PhrG to RapG prevents it from binding DegU PubMed

• Interactions:
  • DegU-RapG PubMed
  • PhrG-RapG PubMed

• Localization:

Database entries

• BsubCyc: BSU40300

• Structure:

• UniProt: O32294

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapG-phrG PubMed

• Expression browser: rapG PubMed

• Sigma factor: SigA PubMed, SigH PubMed

• Regulation:
  • repressed by SinR PubMed
  • repressed by RghR PubMed

• Regulatory mechanism:
  • SinR: transcription repression PubMed
  • RghR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kentaro Hayashi, Tsukahara Kensuke, Kazuo Kobayashi, Naotake Ogasawara, Mitsuo Ogura
Bacillus subtilis RghR (YvaN) represses rapG and rapH, which encode inhibitors of expression of the srfA operon.
Mol Microbiol: 2006, 59(6);1714-29
[PubMed:16553878] [WorldCat.org] [DOI] (P p)

Frances Chu, Daniel B Kearns, Steven S Branda, Roberto Kolter, Richard Losick
Targets of the master regulator of biofilm formation in Bacillus subtilis.
Mol Microbiol: 2006, 59(4);1216-28
[PubMed:16430695] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Kana Shimane, Kei Asai, Naotake Ogasawara, Teruo Tanaka
Binding of response regulator DegU to the aprE promoter is inhibited by RapG, which is counteracted by extracellular PhrG in Bacillus subtilis.
Mol Microbiol: 2003, 49(6);1685-97
[PubMed:12950930] [WorldCat.org] [DOI] (P p)