DnaE

• Description: DNA polymerase III (alpha subunit), part of the replisome
  
  

Gene name	dnaE
Synonyms
Essential	yes PubMed
Product	DNA polymerase III (alpha subunit)
Function	DNA replication
Gene expression levels in SubtiExpress: dnaE
Interactions involving this protein in SubtInteract: DnaE
MW, pI	125 kDa, 6.02
Gene length, protein length	3345 bp, 1115 aa
Immediate neighbours	ytsJ, ytrH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU29230

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
  • required for bacteriophage SPP1 replication PubMed

• Protein family: DnaE subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • DnaE-MutL PubMed
  • DnaE-MutS PubMed
  • DnaE-DnaN PubMed
  • DnaE-SsbA PubMed
  • DnaE-DnaC PubMed
  • DnaE-DnaG PubMed
  • DnaE-HolA PubMed
  • DnaE-PolC PubMed

• Localization:
  • forms foci, the foci dissappear after the formation of DNA mismatches in a MutS-dependent manner or after DNA replication arrest PubMed
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34623

• KEGG entry: [3]

• E.C. number: 2.7.7.7

Expression and regulation

• Operon: dnaE (according to DBTBS)

• Expression browser: dnaE PubMed

• Sigma factor:

• Regulation:
  • induced by DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)