yngE

168

methylcrotonoyl-CoA carboxylase, subunit

Locus: BSU_18210

Molecular weight: 66.13 kDa

Isoelectric point: 8.36

Protein length: 511 aa

Gene length: 1536 bp

Function: mother cell metabolism, leucine utilization

Product: methylcrotonoyl-CoA carboxylase, subunit

Essential: no

E.C.: 6.4.1.4

Synonyms: yngE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4799 (Galperin et al., 2021)

Gene

Coordinates: 1,949,682  1,951,217

The protein

Catalyzed reaction/ biological activity

• 3-methylbut-2-enoyl-CoA + Biotin-CO₂ -- 3-methyl-glutaconyl-CoA +  biotin PubMed

Protein family

• AccD/PCCB family (with AccD and YqjD, according to UniProt)

Domains

• CoA carboxyltransferase N-terminal domain (aa 2-254) (according to UniProt)
• CoA carboxyltransferase C-terminal domain (aa 260-506) (according to UniProt)

Structure

• 1VRG (PDB) (from Thermotoga maritima, 40% identity)
• O31825 (AlphaFold)

Paralogous protein(s)

• YqjD

Expression and Regulation

Operons

• Genes: yngJ-yngI-yngH-yngHB-yngG-yngF-yngE

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigE) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-B081 (yngE::erm), available at the NBRP B. subtilis, Japan
• BKE18210 (yngE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATAATCCATCAGCATTGCCT,  downstream forward: _UP4_TAAAAGCAAGAACTTAGGAG
• BKK18210 (yngE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATAATCCATCAGCATTGCCT,  downstream forward: _UP4_TAAAAGCAAGAACTTAGGAG

References

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