CotY

• Description: spore coat protein (insoluble fraction), necessary for the assembly of the spore crust, the outermost layer of the spore coat
  
  

Gene name	cotY
Synonyms
Essential	no
Product	spore crust protein (insoluble fraction)
Function	spore coat assembly
Gene expression levels in SubtiExpress: cotY
Interactions involving this protein in SubtInteract: CotY
MW, pI	17 kDa, 4.857
Gene length, protein length	486 bp, 162 aa
Immediate neighbours	cotZ, cotX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

GerE regulon, GerR regulon, SigK regulon

The gene

Basic information

• Locus tag: BSU11750

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • homotypic interaction PubMed
  • CotY-CotZ PubMed

• Localization:
  • spore crust PubMed
  • localization requires CotZ PubMed

Database entries

• Structure:

• UniProt: Q08311

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotY-cotZ PubMed

• Expression browser: cotY PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK) PubMed

• Regulatory mechanism:
  • GerE: transcription activation PubMed
  • GerR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Peter T McKenney, Adam Driks, Patrick Eichenberger
The Bacillus subtilis endospore: assembly and functions of the multilayered coat.
Nat Rev Microbiol: 2013, 11(1);33-44
[PubMed:23202530] [WorldCat.org] [DOI] (I p)

Original publications

Daisuke Imamura, Ritsuko Kuwana, Hiromu Takamatsu, Kazuhito Watabe
Proteins involved in formation of the outermost layer of Bacillus subtilis spores.
J Bacteriol: 2011, 193(16);4075-80
[PubMed:21665972] [WorldCat.org] [DOI] (I p)

Peter T McKenney, Adam Driks, Haig A Eskandarian, Paul Grabowski, Jonathan Guberman, Katherine H Wang, Zemer Gitai, Patrick Eichenberger
A distance-weighted interaction map reveals a previously uncharacterized layer of the Bacillus subtilis spore coat.
Curr Biol: 2010, 20(10);934-8
[PubMed:20451384] [WorldCat.org] [DOI] (I p)

Daniela Krajcíková, Magda Lukácová, Denisa Müllerová, Simon M Cutting, Imrich Barák
Searching for protein-protein interactions within the Bacillus subtilis spore coat.
J Bacteriol: 2009, 191(10);3212-9
[PubMed:19304857] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Ritsuko Kuwana, Takashi Okumura, Hiromu Takamatsu, Kazuhito Watabe
The ylbO gene product of Bacillus subtilis is involved in the coat development and lysozyme resistance of spore.
FEMS Microbiol Lett: 2005, 242(1);51-7
[PubMed:15621419] [WorldCat.org] [DOI] (P p)

J Zhang, H Ichikawa, R Halberg, L Kroos, A I Aronson
Regulation of the transcription of a cluster of Bacillus subtilis spore coat genes.
J Mol Biol: 1994, 240(5);405-15
[PubMed:7519271] [WorldCat.org] [DOI] (P p)